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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

V600E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KIAA1549_BRAF

Plasmid

#205838

Purpose

Express mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KIAA1549_BRAF (KIAA1549, BRAF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-OGG1

Plasmid

#215135

Purpose

Expresses mCherry-OGG1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

OGG1 (OGG1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-APEX1

Plasmid

#215126

Purpose

Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APEX1 (APEX1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV uN2C GFP (Ctl)

Plasmid

#224355

Purpose

Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

Depositor

Nicolas Charlet-Berguerand

Article

Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

Insert

uN2C

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Codon-optimized for expression in human, so no pu…

Promoter

CMV + chimeric intyron (CAG)

Available Since

Oct. 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-FEN1

Plasmid

#215129

Purpose

Expresses mCherry-FEN1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FEN1 (FEN1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ARID1A-deltaIDR1-FLAG

Plasmid

#242210

Purpose

Expression of human ARID1A ∆IDR1 (deletion of a.a. 2–970) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted IDR1 from ARID1A (deletion of a.a. 2–970)

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EDAR-COMP5AP-AviTag-9xHis

Plasmid

#157119

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EDAR (EDAR Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-NLS-HA-TurboID-PCNA

Plasmid

#215076

Purpose

Expresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Lentiviral

Tags

NLS-HA-TurboID

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216251

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgSEC61B.N1 (SEC61B Human)

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ARID1A-FLAG

Plasmid

#242209

Purpose

Expression of full-length human ARID1A using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

Plasmid

#159697

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pE-N1-RDH11

Plasmid

#161916

Purpose

Expresses untagged human RDH11. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Retinol dehydrogenase 11 (RDH11 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-RsACE2

Plasmid

#159665

Purpose

Expresses Rhinolophus sinicus ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-BAI3

Plasmid

#116716

Purpose

Lentiviral expression of BAI3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BAI3 (BAI3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

Plasmid

#73208

Purpose

Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

Insert

COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)

Tags

The FAP and mCerulean3 are fused with 2 copies of…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G469V

Plasmid

#82833

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G469V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-SOK

Plasmid

#20327

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRF4_WT

Plasmid

#81782

Purpose

Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-HMBG1

Plasmid

#215130

Purpose

Expresses mCherry-HMBG1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

HMGB1 (HMGB1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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