We narrowed to 8,320 results for: Set

1. pLV-UbC-Tet1c-dCas9

   Plasmid

   #232178

   Purpose

   Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV(MinDis).iGluSnFR

   Plasmid

   #41732

   Purpose

   Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   Myc, PDGFR transmembrane helix (aa 513-561), and …

   Expression

   Mammalian

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   CMV

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CLYBL-TO-hNIL-BSD-mApple

   Plasmid

   #124230

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Insert

   NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

   Plasmid

   #229678

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold, HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HR_G3BP1-V5-APEX2-GFP

   Plasmid

   #105284

   Purpose

   homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
   

   Use

   Human homologous recombination donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-TetOne-Puro-MCL1

   Plasmid

   #202663

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

   Plasmid

   #229676

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Halo, 3xALFA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3.1-nAChr-beta3-YFP

   Plasmid

   #50484

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   YFP fusion in M3-M4 loop (after residue P379). O…

   Promoter

   CMV

   Available since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

9. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

   Plasmid

   #229677

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   SNAP, 3xV5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO_HA.V5.FLAG_NGFR

    Plasmid

    #158250

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.V5.FLAG

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEJS1089: mini-AAV.sgRNA.Nme2Cas9

    Plasmid

    #159536

    Purpose

    Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 with single guide RNA cassette
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. U6-PGK-hCD2

    Plasmid

    #224294

    Purpose

    Expresses sgRNA from U6 promoter and human CD2 under PGK

    Depositor

    Nikhil Joshi

    Article

    Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

    Insert

    U6 sgRNA cassette with CD2 under PGK promoter (CD2 Human)
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6/PGK

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

    Plasmid

    #154781

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    CRFR1 (Crhr1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

    Plasmid

    #154780

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    CRFR1 (Crhr1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid

    #171814

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-VHC

    Plasmid

    #112618

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLH_Scr15

    Plasmid

    #100537

    Purpose

    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    CreC, CreN, and SV40 NLS

    Expression

    Yeast

    Mutation

    N-terminal version of PhyB (AA 1-621)/ Mutation o…

    Promoter

    FBA1 and TDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-tdTHC

    Plasmid

    #112621

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid

    #111390

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    ChETA-mRuby2
    

    Use

    AAV

    Tags

    Myc and mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-mTHC

    Plasmid

    #112620

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    mTeal (mTFP1)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO_S.AU1.VSVg_NGFR

    Plasmid

    #158351

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.AU1.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLH_Scr16

    Plasmid

    #100538

    Purpose

    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Cdc1 NLS, CreC, CreN, and SV40 NLS

    Expression

    Yeast

    Mutation

    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A

    Promoter

    FBA1 and TDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid

    #111391

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA-mRuby2
    

    Use

    AAV

    Tags

    Myc and mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid

    #156452

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG

    Depositor

    Elphege Nora

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    RAD21, AID (Rad21 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge