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Showing: 5421 - 5440 of 7989 results

  1. pD649-HAsp-FCRL6-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156729
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCRL6 (FCRL6 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156729-plasmid-map-sequence-id-393041

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  3. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  5. hsp70: mKate2 dynactin1-811

    Plasmid
    #105970
    Purpose
    heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    dynactin1-811 (DCTN1 Human)
    Tags
    mKate2
    Expression
    Bacterial
    Mutation
    contains only amino acids 1-811
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    105970-plasmid-map-sequence-id-203781

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  12. pBIG1A.HMBP.PrS.PRC2-trunc

    Plasmid
    #247015
    Purpose
    Expression of EZH2, SUZ12 (70-685), EED (80-411), RBBP4 under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Inserts
    EZH2 (EZH2 Human)
    SUZ12 (SUZ12 Human)
    EED (EED Human)
    RBBP4 (RBBP4 Human)
    Tags
    MBP
    Expression
    Insect
    Mutation
    aa 70-685 only and aa 80-411 only
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247015-plasmid-map-sequence-id-493377

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  16. hnRNPA1_allW_D262V-FLAG-IRES-eGFP

    Plasmid
    #234621
    Purpose
    Mammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1 allW D262V (HNRNPA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Famillial ALS mutation D262V, all aromatic amino …
    Promoter
    CMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234621-plasmid-map-sequence-id-464954

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Showing: 5421 - 5440 of 7989 results