We narrowed to 9,109 results for: control

1. p23-mNeongreen-DDX21 DAT

   Plasmid

   #179229

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mNeongreen

   Expression

   Mammalian

   Mutation

   G234D, K235A

   Promoter

   EF-1alpha

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLY54

   Plasmid

   #130923

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1 hSlp4-a C2AB

   Plasmid

   #40037

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp4-a C2AB (SYTL4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C2A and C2B domains (353-671)

   Promoter

   CMV promoter

   Available since

   Oct. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 hSlp4-a C2A

   Plasmid

   #40038

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp4-a C2A (SYTL4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C2A domain (353-487)

   Promoter

   CMV promoter

   Available since

   Oct. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

   Plasmid

   #112762

   Purpose

   express GFP-4EBP1-Rheb15(CAAX mutation)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
   

   Use

   Lentiviral

   Tags

   Rheb15(CAAX mutant)

   Expression

   Mutation

   CAAX mutation to prevent lipid localization

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRY2low-mCherry-Raf1

   Plasmid

   #104066

   Purpose

   Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2low-mCherry-Raf1 (CRY2, RAF1 Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2 aa 1-491 truncation, R489E A491D

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV superYFP-AURKA K162M-mTurq2

   Plasmid

   #157773

   Purpose

   Expression of AuroraA kinase-dead biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   mTurquoise2 and superYFP

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Flag-YAP-S381A

   Plasmid

   #33090

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S381A

   Promoter

   CMV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-C1 hSlp4-a C2B

   Plasmid

   #40039

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp4-a C2B (SYTL4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C2B domain (488-671)

   Promoter

   CMV promoter

   Available since

   Oct. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX4T-1 hSlp2-a del.C2AB

    Plasmid

    #40059

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a del.C2AB (SYTL2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion of C2A and C2B domains (601-910)

    Promoter

    Tac

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-APLP1ICD-IRES-hrGFP

    Plasmid

    #107546

    Purpose

    AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    APLP1ICD (APLP1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LADL Bridge + Promoter Only Target

    Plasmid

    #127669

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-hs-3xHA-NHL only-LIN-41

    Plasmid

    #52723

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    NHL-only contains only an initiating methionine a…

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-hs-3xHA-7CtoA-LIN-41

    Plasmid

    #52726

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed cysteines 12, 15, 61, 66, 69, 91, and 94 …

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1 hSlp2-a V18A

    Plasmid

    #40054

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a V18A (SYTL2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Valine 18 to Alanine

    Promoter

    CMV promoter

    Available since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-C1 Slp2-a C2B

    Plasmid

    #40053

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a C2B (Sytl2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2B domain

    Promoter

    CMV promoter

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-CARMA2sh-E142G

    Plasmid

    #97070

    Purpose

    Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamic Acid 142 to Glycine

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTH752-CEN-RLuc/min103maxCFLuc

    Plasmid

    #38218

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 103 codons of the original FLuc gene we…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTH751-CEN-RLuc/min53maxCFLuc

    Plasmid

    #38217

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 53 codons of the original FLuc gene wer…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX4T-1 Slp2-a E11A/R32A

    Plasmid

    #40063

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a E11A/R32A (Sytl2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Glutamate 11 to Alanine, Arginine 32 to Alanine

    Promoter

    Tac

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only