We narrowed to 19,432 results for: INO

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pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

Plasmid

#175801

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

AviTag, Hexahistidine tag, and eGFP

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUb-G76V-GFP-IRES-DsRed

Plasmid

#225503

Purpose

Expression of Ub-G76V-GFP along dsRED from one mRNA

Depositor

David Rubinsztein

Article

Yu et al Hum Mol Genet. 2024 May 22:ddae085. doi:

Insert

Ub-G76V-GFP

Tags

GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR1 (Retro)

Plasmid

#222687

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(wt)

Plasmid

#87929

Purpose

Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDIO-DSE-mCherry-PSE-MCS

Plasmid

#129669

Purpose

Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

Depositor

Beatriz Rico

Article

Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

Type

Empty backbone

Use

AAV

Tags

mCherry

Promoter

EF1a

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6*)-mCherry

Plasmid

#168048

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101* and PBAD

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT sfGPF 150TAG

Plasmid

#154766

Purpose

plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in GFP reporter, U25C in PylT

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe B-Raf V600E

Plasmid

#17544

Depositor

Channing Der

Article

Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

Insert

B-Raf (BRAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

V600E

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-GBF1

Plasmid

#206415

Purpose

Mammalian expression of hGBF1

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CD2APtk

Plasmid

#86004

Purpose

homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

DsRedEx_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPBbleo-ErbB1

Plasmid

#197359

Purpose

Encoding human ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-Mx1201 PylRS

Plasmid

#154764

Purpose

plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pBa.TfR-Halo

Plasmid

#162716

Purpose

Mammalian expression of human TFRC

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

TfR (TFRC Human) (TFRC Human)

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-p14

Plasmid

#42330

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p14 (LAMTOR2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR4 (Retro)

Plasmid

#222690

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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p23-NES-RfxCas13d-msfGFP-NES-Flag

Plasmid

#165076

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDule-pCNF

Plasmid

#85494

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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HA WDR24 pRK5

Plasmid

#46335

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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