We narrowed to 9,184 results for: Pol;

1. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-mir-17-92

   Plasmid

   #64100

   Purpose

   expresses mir-17-92

   Depositor

   Lin He

   Article

   Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

   Insert

   miR-17-92 (MIR17HG Human)
   

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P-1-eIF4E K119A

   Plasmid

   #199440

   Purpose

   Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

   Depositor

   Shin-ichi Hoshino

   Article

   Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

   Insert

   1977 (EIF4E Human)
   

   Tags

   GST-fusion protein

   Expression

   Bacterial

   Mutation

   changed lysine 119 to alanine

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RDX-mCherry

   Plasmid

   #214476

   Purpose

   Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   RDX (RDX Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Sequence matches cDNA clone MGC:48283 IMAGE:52844…

   Promoter

   CMV

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQHA-USP11 WT puroR

   Plasmid

   #46749

   Purpose

   Retroviral vector that expresses wild type HA-tagged wild type human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Hyg-Dest-mCherry-PAR-6B

   Plasmid

   #194908

   Purpose

   To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   PAR-6B (Pard6b Mouse)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   39 C to G, silent mutation

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-AN-HA_Ubiquitin

   Plasmid

   #131258

   Purpose

   Mammalian expression of ubiquitin with an N-terminal HA tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SYN-H-sDarken

   Plasmid

   #184797

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TetO-FUW-PBRM1-pgk-puro

   Plasmid

   #85746

   Purpose

   tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

   Depositor

   Emily Dykhuizen

   Article

   Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   none

   Promoter

   CMV

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQHA-USP11 CS puroR

    Plasmid

    #46750

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR651

    Plasmid

    #65213

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-1 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. W118-1_hTGM3-Flag

    Plasmid

    #180408

    Purpose

    lentiviral transduction of human TGM3 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM3 (TGM3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330 Human 3' HP1a gRNA

    Plasmid

    #127907

    Purpose

    WT Cas9 Vector targeting the 3' end of the human HP1a gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    gRNA for Human 3' HP1a
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid

    #119868

    Purpose

    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-OT (wt TCF-4 sites)

    Plasmid

    #16558

    Depositor

    Bert Vogelstein

    Article

    Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

    Insert

    TCF-4 binding sites (TCF4 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    3 copies of wildtype Tcf-4 binding sites

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-DDK_WT RAD23B

    Plasmid

    #201439

    Purpose

    Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-SYN-L-sDarken

    Plasmid

    #184798

    Purpose

    Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    L-sDarken
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. W118-1_hTGM4-Flag

    Plasmid

    #180409

    Purpose

    lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM4 (TGM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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