We narrowed to 7,184 results for: RAP

1. pAAV-CAG-miRFP2

   Plasmid

   #178968

   Purpose

   Expresses miRFP2 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   miRFP2
   

   Use

   AAV

   Tags

   no

   Expression

   Mammalian

   Mutation

   NO

   Promoter

   CAG

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

   Plasmid

   #194463

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Emerald-MannII-N-10

   Plasmid

   #54150

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   MannII (Man2a1 Mouse)
   

   Use

   Tags

   Emerald

   Expression

   Mammalian

   Mutation

   aa 1-112 of MannII aa 1-112 of MannII

   Promoter

   CMV

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pAAV_K700E_hSF3B1

   Plasmid

   #176591

   Purpose

   Adenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1

   Depositor

   Manoj Pillai

   Article

   Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

   Insert

   SF3B1 (SF3B1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-RNF8-C-terminus

   Plasmid

   #64673

   Purpose

   expresses only C terminus of RNF8, amino acids 402-485

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   Ring finger protein 8 (RNF8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   containing only C-terminus amino acids 402 to 485

   Promoter

   CMV

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. JPF0535a

   Plasmid

   #124042

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG416GALL-HAV_3C

   Plasmid

   #203481

   Purpose

   Expresses HAV 3C protease from a GALL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   HAV 3C protease
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GALL

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-Pcdh15_mini_V7-CD2

   Plasmid

   #199186

   Purpose

   An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

   Depositor

   David Corey

   Article

   Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

   Insert

   mmPcdh15_mini_V7-CD2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   EC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-Pcdh15_mini_V8-CD2

   Plasmid

   #199187

   Purpose

   An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

   Depositor

   David Corey

   Article

   Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

   Insert

   mmPcdh15_mini_V8-CD2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid

    #62133

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    human codon optimized Cas9
    

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mKate2-P2A-APEX2-2xML1N(7Q)

    Plasmid

    #67666

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1Nmut*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL-p21UTRm1

    Plasmid

    #20878

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pSpCas9-Puro HLAG-2B-sgRNA

    Plasmid

    #182552

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2B-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FusXTBE-1397-C

    Plasmid

    #201398

    Purpose

    Expresses C terminus half of DddAtox in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FusXTBE-1397-N

    Plasmid

    #201397

    Purpose

    Expresses N terminus half of DddAtox in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

    Plasmid

    #105192

    Purpose

    Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZIKV_NASBA_32B

    Plasmid

    #75011

    Purpose

    Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_32B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. P1_Firre_1.49_pDONR221

    Plasmid

    #195204

    Purpose

    Plasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.

    Depositor

    John Rinn

    Article

    Hacisuleyman et al Nat Struct Mol Biol. 2014 Feb;21(2):198-

    Insert

    mFirre 1.49 Clone
    

    Use

    Gateway cloning intermediate

    Tags

    Expression

    Mutation

    Only exons

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR221-TAZ CA

    Plasmid

    #79504

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3xFLAG

    Expression

    Mutation

    S89A

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3xFLAG-CMV-RNF8-N-terminus(1-140)

    Plasmid

    #64675

    Purpose

    expresses only N terminus of RNF8, amino acids 1 to 140

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    only N terminus amino acids 1-140

    Promoter

    CMV

    Available since

    May 20, 2015

    Availability

    Academic Institutions and Nonprofits only