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pRMH124-VP16-CIB1

Plasmid

#40615

Purpose

Expresses VP16-CIB1 for light-inducible protein expression when coupled with GalBD-CRY2PHR or GalBD-CRY2

Depositor

Chandra Tucker

Article

Hughes et al Methods. 2012 Aug 15.

Insert

VP16-CIB1

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pGST-Sp1

Plasmid

#27264

Depositor

Thomas Gilmore

Article

Sif et al J Virol. 1994 Nov . 68(11):7131-8.

Insert

Sp1 (SP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 83-621

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pPBbsr-JNK KTR-mCherry

Plasmid

#115493

Purpose

Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

JNK KTR-mCherry

Tags

mCherry

Expression

Mammalian

Mutation

mCherry G229L, mCherry Y298 deleted

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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AMPK-β2-FLAG

Plasmid

#40603

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
MigR1-AE9aP

Plasmid

#12434

Depositor

Dong-Er Zhang

Article

Yan et al Nat Med. 2006 Aug . 12(8):945-9.

Insert

AML1-ETO9a as in t(8;21) patients (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

AML1-ETO9a cDNA as in t(8;21) patients

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pHV738

Plasmid

#117476

Purpose

Expression of NMT1

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

NMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMM136

Plasmid

#127217

Purpose

BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, MP-delta, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMF1754

Plasmid

#125585

Purpose

Expresses peroxisomal KillerRed (po-KR) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

po-KillerRed

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-p2A-CreERT2-FRT-kan-FRT

Plasmid

#108454

Purpose

This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

mCherry-p2a-CreERT2

Use

Cre/Lox

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowG

Plasmid

#104620

Purpose

Expresses Shadow G in mammalian cell

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

Insert

ShadowG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pUPRT::DHFR-D

Plasmid

#58528

Purpose

replace TgUPRT with DHFR by homologous recombination

Depositor

David Sibley

Article

Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

Insert

DHFR cassette

Use

Gateway Cloning

Mutation

pyrimethamine resistant

Promoter

DHFR

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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GST-PRMT2

Plasmid

#34650

Depositor

Steven Clarke

Article

Miranda et al Biochem Biophys Res Commun. 2005 Oct 28;

Insert

PRMT2 (PRMT2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC16A1

Plasmid

#131934

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A1 (SLC16A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP Tensin3

Plasmid

#105299

Purpose

To study localization and function of Tensin3

Depositor

David Critchley, Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin3 (TNS3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-N2cG

Plasmid

#100811

Purpose

express rabies virus CVS N2c strain glycoprotein gene (N2cG)

Depositor

Ian Wickersham

Insert

rabies virus CVS N2c strain glycoprotein gene

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAM1573

Plasmid

#40239

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Cm Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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p5194 pGL4.20 HPV18-LCR-luciferase

Plasmid

#22859

Depositor

Peter Howley

Article

Schweiger et al J Virol. 2007 Sep . 81(18):9612-22.

Insert

HPV18-LCR-luciferase

Expression

Mammalian

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pSG5 Large T K1

Plasmid

#9055

Depositor

William Hahn

Insert

large T antigen K1 (SV40gp6 simian virus)

Expression

Mammalian

Mutation

E107K. Mutation in pRb LxCxE binding domain.

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
iRFP-Sec61beta

Plasmid

#108125

Purpose

ER marker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

iRFP:SEC61B (SEC61B Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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