We narrowed to 9,825 results for: 158

1. HR700_HA2TP53_Ex5

   Plasmid

   #228860

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. HR700_HA2TP53_Ex6

   Plasmid

   #228861

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. HR700_HA2TP53_Ex7

   Plasmid

   #228862

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. HR700_HA2TP53_Ex8

   Plasmid

   #228863

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

   Plasmid

   #231206

   Purpose

   Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L147I, R148H, I203V, L290V

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcs2-cDELE1(142-515)-sumo

   Plasmid

   #226107

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Use

   Tags

   Sumo

   Expression

   Mammalian

   Mutation

   encodes only residues 142-515 of DELE1 (represent…

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcs2-MTS-cox8A-GFP-IRES-mCherry

   Plasmid

   #226103

   Purpose

   Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   COX8A (COX8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   encodes only for mitochondrial targeting sequence…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcs2-cDELE1(142-515)-GFP-IRES-mCherry

   Plasmid

   #226102

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only encodes for residues 142-515 of DELE1 corres…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-CRT-Fc P

   Plasmid

   #221855

   Purpose

   Express human Calreticulin truncated P domain with C-terminal human IgG1Fc tag in mammalian cell.

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

   Insert

   Calreticulin (CALR Human)
   

   Use

   Tags

   Human IgG1Fc tag

   Expression

   Mammalian

   Mutation

   Expresses P domain

   Promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-CRT-Fc N

    Plasmid

    #221854

    Purpose

    Express human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses N domain

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-CRT-Fc C

    Plasmid

    #221856

    Purpose

    Express human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses C domain

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LT3GEPIR-Rb1shRNA1

    Plasmid

    #220584

    Purpose

    To inducibly knockdown Rb1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    Rb1 shRNA1 (RB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LT3GEPIR-Rb1shRNA2

    Plasmid

    #220585

    Purpose

    To inducibly knockdown Rb1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    Rb1 shRNA2 (RB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LT3GEPIR-Rb1shRNA3

    Plasmid

    #220586

    Purpose

    To inducibly knockdown Rb1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    Rb1 shRNA3 (RB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_003.gEZH2

    Plasmid

    #220318

    Purpose

    Expresses guide RNA for CRISPR/Cas9 knockout of EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 gRNA (EZH2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHIV-EGFP.Flag-EZH2-mt1

    Plasmid

    #220244

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R27A, R31A, F165A

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFB1.HMBP.PrS.EZH2-mt1

    Plasmid

    #220239

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    R27A, R31A, F165A

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

    Plasmid

    #202064

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-ZNF473KRAB-Citrine
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITR deletion

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT312 AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator

    Plasmid

    #202061

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITRs intact

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid

    #208966

    Purpose

    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-PDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA

    Plasmid

    #210502

    Purpose

    expresses LAUNCHERtvmv component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    PDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid

    #208973

    Purpose

    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUt-mNF-GFP

    Plasmid

    #199220

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCW57.1-Gata6-3xT7

    Plasmid

    #200889

    Purpose

    Inducible lentiviral expression of Gata6

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSI-218

    Plasmid

    #197139

    Purpose

    All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SpCas9-PmCDA1-UGI

    Expression

    Mammalian

    Mutation

    SpCas9(D10A)

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

    Plasmid

    #148514

    Purpose

    Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

    Plasmid

    #148515

    Purpose

    Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T

    Plasmid

    #147590

    Purpose

    Insect Expression of DmThor-CG32016-E

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-CG32016-E (Thor Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    DmThor two silent mutations compared to the seque…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T

    Plasmid

    #147591

    Purpose

    Insect Expression of DmThor-DmCup_417-1133

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-DmCup_417-1133 (Thor Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Thor contains two silent mutations A246C and T321…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge