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173,219 results

Showing: 10961 - 11000 of 173219 results

  1. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  2. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124607-plasmid-map-sequence-id-244336

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  12. pNL [NlucP/TCF/LEF-RE/Hygro] Vector

    Plasmid
    #236824
    Purpose
    Express LEF response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    LEF
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236824-plasmid-map-sequence-id-510767

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  14. HypoxCR

    Plasmid
    #59946
    Purpose
    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.
    Depositor
    Anne Le
    Article
    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p
    Inserts
    GFP-Pest
    mCherry-Geminin
    Use
    Lentiviral
    Tags
    FLAG and PEST
    Expression
    Mammalian
    Promoter
    CMV and min CMV
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    59946-plasmid-map-sequence-id-228094

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  16. SARS-CoV-2 S HexaPro

    Plasmid
    #154754
    Purpose
    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
    Depositor
    Jason McLellan
    Article
    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505
    Insert
    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Available Since
    July 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154754-plasmid-map-sequence-id-304640

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  19. pcDNA3.1.Gα11-LgB97

    Plasmid
    #134361
    Purpose
    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα11-LgB97 (GNA11 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134361-plasmid-map-sequence-id-306399

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  26. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα13-LgB106 (GNA13 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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  30. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

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  31. pHIV-Luciferase

    Plasmid
    #21375
    Purpose
    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21375-plasmid-map-sequence-id-357945

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  37. mTLR4

    Plasmid
    #13085
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (Tlr4 Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13085-plasmid-map-sequence-id-142564

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Showing: 10961 - 11000 of 173219 results