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159,117 results

Showing: 10961 - 11000 of 159117 results

  1. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

    Plasmid
    #186046
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8s
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186046-plasmid-map-sequence-id-370170

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  3. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  14. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181743-plasmid-map-sequence-id-354675

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  26. pLSLGFP.R

    Plasmid
    #51501
    Purpose
    Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Insert
    GFP
    Use
    Plant t-dna plasmid
    Tags
    Expression
    Mutation
    Promoter
    2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51501-plasmid-map-sequence-id-261239

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  27. pET28a-UBC9

    Plasmid
    #228949
    Purpose
    express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228949-plasmid-map-sequence-id-472392

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  28. pET28a-UBC9-K14R-A129K

    Plasmid
    #228948
    Purpose
    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Mutation
    changed Lysine 14 to Arginine, changed Alanine 12…
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228948-plasmid-map-sequence-id-472389

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  29. TFORF1817

    Plasmid
    #143981
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    UHRF1 (UHRF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    143981-plasmid-map-sequence-id-471145

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  31. pAIP4_4WDC

    Plasmid
    #234153
    Purpose
    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4WDC
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234153-plasmid-map-sequence-id-464119

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  32. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid
    #232344
    Purpose
    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag
    Depositor
    Philip Wong
    Article
    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10
    Insert
    HDGF Like 2 (HDGFL2 Human)
    Use
    Tags
    GGGS linker, 6xHis and Twin-strep, TEV protease s…
    Expression
    Mammalian
    Mutation
    TDP-43 regulated cryptic exon
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232344-plasmid-map-sequence-id-462328

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  34. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Article
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Use
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

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  36. pBAD-luxKOk

    Plasmid
    #229944
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr
    Insert
    luxKOk
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229944-plasmid-map-sequence-id-457161

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  37. pBAD-luxGold

    Plasmid
    #229943
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr
    Insert
    luxGold
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229943-plasmid-map-sequence-id-457160

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Showing: 10961 - 11000 of 159117 results