161,273 results

1. pET16b-His-ArgRS

   Plasmid

   #124104

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Argin+I4 ligase (argS Escherichia coli)
   

   Use

   Tags

   10x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAVS1-P-CAG-DEST

   Plasmid

   #80490

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQE30-His-AsnRS

   Plasmid

   #124105

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Asparaginyl-tRNA ligase (asnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Dronpa3-N1

   Plasmid

   #54682

   Purpose

   Localization: N1 Cloning Vector, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   Dronpa3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pLenti-hACE2-hygro

   Plasmid

   #161758

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pS-cr:GFP

   Plasmid

   #196295

   Purpose

   35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
   

   Use

   CRISPR

   Tags

   SpeI-DR-BsaI-DR-SpeI

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHis-MBP-huMb3Cas12a

   Plasmid

   #174677

   Purpose

   Expresses 6xHis-MBP tagged Mb3Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb3Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis-MBP-huMb2Cas12a

   Plasmid

   #174676

   Purpose

   Expresses 6xHis-MBP tagged Mb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRN3P-HA-Suv4-20h2mut

   Plasmid

   #86692

   Purpose

   For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Mutated asparagine 273 to cysteine 276 (NHDC) int…

   Promoter

   T3

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SnoopTagJr-MBP

    Plasmid

    #105628

    Purpose

    Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SnoopTagJr-MBP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Htr5b(3.7)-tTA-sv40

    Plasmid

    #184412

    Purpose

    AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-tTA
    

    Use

    AAV

    Tags

    tTA

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PTAL10

    Plasmid

    #60671

    Purpose

    two TALEN monomers in one plasmid

    Depositor

    Renzhi Han

    Article

    Mariano et al BMC Res Notes. 2014 Sep 10;7:628. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid

    #181751

    Purpose

    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLVgag-3xNES-ABE8e
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. TCR012H

    Plasmid

    #233454

    Purpose

    Expresses ncHLAp-reactive T cell receptor (TCR012H)

    Depositor

    William Freed-Pastor

    Article

    Ely et al Science. 2025. May 8; 388(6747)

    Insert

    TCR012H
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-SpyTag-Flag-Lbr-V5-mCherry

    Plasmid

    #235094

    Purpose

    Lbr mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-Lbr-V5-mCherry (LBR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut

    Plasmid

    #235590

    Purpose

    Dox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NHDC182-185AAAG

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti Puro HA-Ubiquitin-K48

    Plasmid

    #236290

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Ubiquitin K48 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Single ubiquitin coding sequence containing K48 m…

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLenti Puro HA-Ubiquitin-K63

    Plasmid

    #236291

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Ubiquitin K63 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Single ubiquitin coding sequence containing K63 m…

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pZH81 pET21b Rat1-His

    Plasmid

    #231650

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    Rat1 (RAT1 Budding Yeast)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZH82 pET28a-Rai1

    Plasmid

    #231651

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    rai1 (RAI1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pZH23 Dual G-less containing ySSA1 polyA

    Plasmid

    #231647

    Purpose

    yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    SSA1 polyA termination region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZH20 Dual G-less containing yCYC1 polyA

    Plasmid

    #231646

    Purpose

    yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    CYC1 polyA termination region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pB-3xFlag-dCas13-mTET2HxDCD

    Plasmid

    #228235

    Purpose

    For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

    Depositor

    Chuan He

    Article

    Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

    Inserts

    dCas13
    TET2 (Tet2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    CD (cysteine-rich, dioxygenase domain) truncation…

    Promoter

    CAG

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDM042

    Plasmid

    #216812

    Purpose

    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mScarlet3
    

    Use

    Tags

    VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid

    #214125

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    dCas9-TET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC150-FAM134C-GFP

    Plasmid

    #201932

    Purpose

    PiggyBac Vector expressing FAM134C-GFP

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    FAM134C (RETREG3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP hSmad4-HomDonor

    Plasmid

    #206044

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193315

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Use

    Tags

    4FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMIMY

    Plasmid

    #191954

    Purpose

    A pMSCV-based backbone where the MCS has been substituted with a MCS-IRES-mitoYFP for expression of a gene of interest and a mitochondrially targeted YFP

    Depositor

    Luca Scorrano

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBAD/HisB (TIREVOL)-mNG

    Plasmid

    #189722

    Purpose

    titratable expression of mNeonGreen in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mNeonGreen
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLL5.0 shFmnl2-EGFP

    Plasmid

    #187255

    Purpose

    Lentiviral expression of shRNA targeting Fmnl2

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Fmnl2 (Fmnl2 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJW1816

    Plasmid

    #154320

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. KA0718_pPB-TREtight-cHA-IRESVenus

    Plasmid

    #124171

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RRM2 E4.3 gRNA

    Plasmid

    #90883

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM2 (Guide Designation E4.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RRM2 E3.3 gRNA

    Plasmid

    #90882

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM2 (Guide Designation E3.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AiP15578 - pAAV-AiE0780m-minBG-iCre(R297T)-BGHpA (Alias: CN5578)

    Plasmid

    #224200

    Purpose

    AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lyn FAK biosensor

    Plasmid

    #78299

    Purpose

    FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Lyn Fak biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-N2cG

    Plasmid

    #100811

    Purpose

    express rabies virus CVS N2c strain glycoprotein gene (N2cG)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain glycoprotein gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge