We narrowed to 21,470 results for: SPR

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nSaCas9 I744 miniABEMax V82G

Plasmid

#135379

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 I744 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Cplx1-GFP KI

Plasmid

#131475

Purpose

Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cplx1, Cplx1 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pNS19-LV-mutRFP-2A-GFP

Plasmid

#113716

Purpose

Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

mutRFP-2A-GFP-IRES-Puro

Use

Lentiviral; Reporter system

Expression

Mammalian

Mutation

Y64L (mutRFP)

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC41

Plasmid

#62332

Purpose

sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
PCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

Plasmid

#55199

Purpose

CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

ECFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAG149 NeuroD 3'UTR

Plasmid

#12007

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N14 3'UTR (NEUROD1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pFnCpfAb-apr

Plasmid

#214198

Purpose

Expresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumannii

Depositor

Yu Wang

Article

Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

Insert

FnCpf1

Use

CRISPR

Promoter

tac promoter

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFETCh_ARID5B

Plasmid

#86288

Purpose

Donor vector for 3' FLAG tag of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID5B homology arms (ARID5B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA1_NANOG

Plasmid

#64153

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBFC1171

Plasmid

#231994

Purpose

CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site

Depositor

Brady Cress

Article

Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

Insert

crRNA of dRfxCas13d

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pJEx (Jungle Express)

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

Plasmid

#225753

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pQCascade(Ptr)-entry(BsaI)

Plasmid

#175579

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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5aa SunTag VP64 EVD

Plasmid

#115483

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Promoter

pJ23119

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_KDM1A_1

Plasmid

#86297

Purpose

Encodes gRNA for 3' target of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM1A (KDM1A Human)

Use

CRISPR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x7

Plasmid

#63601

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLV/Guide-mCherry

Plasmid

#170543

Purpose

Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

mCherry

Use

CRISPR and Lentiviral

Promoter

Ef1-alpha

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_2

Plasmid

#101077

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-RHO-IntC-dCas9C-VPR-polyA

Plasmid

#166692

Purpose

Express C-terminal part of split dCas9-VPR

Depositor

Stylianos Michalakis

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Insert

IntC-dCas9C-VPR

Use

AAV and CRISPR

Expression

Mammalian

Promoter

short human rhodopsin promoter

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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