8,922 results

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pMRBad-Z-CspGFP

Plasmid

#40730

Depositor

Brian McNaughton

Article

Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

Insert

Leucine Zipper prime - C super positive Green Fluorescent Protein

Expression

Bacterial

Promoter

pBad

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-SrtAdelta59

Plasmid

#51138

Purpose

expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.aureus SrtA delta 59

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only
pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd (RESCUE)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoSTIM1

Plasmid

#70159

Purpose

Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

Insert

OptoSTIM1 (STIM1 Mustard Weed, Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-SpySrtA

Plasmid

#51139

Purpose

expression plasmid for N-terminal his tagged S.pyogenes SrtA

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.pyogenes SrtA

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-80

Promoter

T7

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Tags

sfCherry11x4

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT WT

Plasmid

#84924

Purpose

luciferase reporter for TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-SADB19L

Plasmid

#32632

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

SADB19L

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pMLRT

Plasmid

#78234

Purpose

Expresses Murine Leukemia Reverse Transcriptase (MLRT)

Depositor

Christopher Voigt

Article

Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

Insert

Murine leukemia reverse transcriptase

Expression

Bacterial

Promoter

J23102

Available Since

Aug. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD wt

Plasmid

#19316

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA

Plasmid

#46759

Purpose

gRNA cloning vector for in vitro transcription of target gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

gRNA core

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai2

Plasmid

#16369

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai2 (ORAI2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flpo

Plasmid

#55637

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV1, and AAV2

Insert

Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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BirA(R118G)-HA destination vector

Plasmid

#53581

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

Depositor

Karl Kramer

Type

Empty backbone

Tags

BirA(R118G) and HA tag

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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CHMP2A_GFP_N_term

Plasmid

#31805

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP2A (CHMP2A Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMVTRE3G eGFP Neo (w821-1)

Plasmid

#27569

Depositor

Eric Campeau

Insert

Enhance Green Fluorescent Protein

Use

Lentiviral

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5-Flag-RhoA wt

Plasmid

#11750

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits