We narrowed to 18,759 results for: nls
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Plasmid#126458PurposeExpresses Cas9-15xFlag-TdT in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only
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pPRISM-Fuse-gcry-TagBFP
Plasmid#117783PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-eGFP
Plasmid#117788PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-gcry-TagBFP
Plasmid#117789PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPRISM-Cre-gcry-mRFP
Plasmid#117790PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPRISM-TagRFP-gcry-mRFP
Plasmid#117766PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-TagBFP
Plasmid#117768PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-TagBFP
Plasmid#117771PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3x3 Stop sequence
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-cmlc2-TagBFP
Plasmid#117774PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3x3 Stop sequence
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-cmlc2-mRFP
Plasmid#117775PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3x3 Stop sequence
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-WT(pL84)
Plasmid#124400PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGal4-TET1-878-911 wildtype (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PrCas12a-2AeGFP
Plasmid#123636PurposeMammalian expression, Genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPrCas12a
Tags3xHA tagExpressionMammalianAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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sgMAPKAP1_1
Plasmid#124912PurposeTargeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9DepositorInsertMAPKAP1 (MAPKAP1 Human)
UseCRISPR and LentiviralAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg1
Plasmid#124856PurposeMutagenesis of Gabrg1DepositorInsertGabrg1 (Gabrg1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria2
Plasmid#124868PurposeMutagenesis of Gria2DepositorInsertGria2 (Gria2 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria1
Plasmid#124867PurposeMutagenesis of Gria1DepositorInsertGria1 (Gria1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-HA-Tbkbp1
Plasmid#125177PurposeRetroviral expression of mouse Tbkbp1DepositorAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
Plasmid#124286PurposesgRNA against human Col7A1DepositorInsertCollagen type VII alpha 1 chain (COL7A1 Human)
UseCRISPRAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C.1.0-gDNA
Plasmid#113792PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2CDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFAP2C (TFAP2C Human)
UseCRISPRExpressionMammalianAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL003
Plasmid#122859PurposeBinary expression vector containing 35S:MS2-GFP:tRBCS followed by GreenGate A-G sequences for insertion of additional modulesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp35S:MS2-GFP:tRBCS
UseGolden gate compatible plant transformation vectorAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL024
Plasmid#122863PurposeBinary expression vector containing 35S:MS2-p300:tRBCS followed by GreenGate A-G sequences for insertion of additional modulesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert35S:MS2-p300:tRBCS
UseGolden gate compatible plant transformation vectorAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1
Plasmid#118169PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118163PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118161PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2
Plasmid#118170PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL036
Plasmid#122864PurposeBinary expression vector containing 35S:MS2-G9a:tRBCS followed by GreenGate A-G sequences for insertion of additional modulesDepositorInsert35S:MS2-G9a:tRBCS
UseGolden gate compatible plant transformation vectorAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH205
Plasmid#122861PurposeBinary expression vector containing pSUC2:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modulesDepositorInsertpSUC2:MS2-VP64:tRBCS
UseGolden gate compatible plant transformation vectorAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-AmCas12b
Plasmid#121951PurposeE. coli expression, protein purificationDepositorInsertAmCas12b
Tags10xHis tagExpressionBacterialAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF713
Plasmid#121665PurposeU6-sgRNA EF1a-ProCas9-FlaviS6-P2A-PuroDepositorInsertsUseLentiviralPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3.-6_human ProCas9Flavi-6
Plasmid#121630PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneExpressionMammalianPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH235
Plasmid#121145PurposeCas9 expression controlled by the nmt41 promoterDepositorInserthumanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastPromoternmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dNmeCas9
Plasmid#120077PurposedNmeCas9 with no sgRNA cassette lenti constructDepositorInsertdNmeCas9
UseLentiviralAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBsCas12b
Tags3xHA tagExpressionMammalianAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b(D977A)-2AeGFP
Plasmid#121962PurposeMammalian expression, Transcription regulation, deactivated AaCas12bDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCatalytically deactivated AaCas12b
Tags3xHA tagExpressionMammalianMutationD977AAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)
Plasmid#120293PurposeAAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffoldDepositorInsertN-terminal fragment of SpyCas9
UseAAV and CRISPRTagsSV40-NLS and split-inteinExpressionMammalianAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
bTub-GFP.766A
Plasmid#112695Purposeexpressed GFP in testes, under bTub promoterDepositorInsertbTub-GFP-SV40
ExpressionInsectPromoterbTubAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCas9-Nours
Plasmid#107733PurposeEscherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiaeDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionYeastMutationHuman optimizedPromoterTEF1Available sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113687PurposeSaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only