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PB-U6insert

Plasmid

#104536

Purpose

A piggybac vector with a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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U6_crRNA_mCherry

Plasmid

#197609

Purpose

Expresses a Cas7-11 crRNA and mCherry

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

U6_crRNA_mCherry

Expression

Mammalian

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAN3938

Plasmid

#74697

Purpose

Circuit plasmid: 0xF6

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0xF6

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet2

Plasmid

#218361

Purpose

Empty backbone with tetR-PtetA expression system with two tetR operator sites

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Cas7-11_T2A_Csx29

Plasmid

#197607

Purpose

Expresses Cas7-11 and Csx29 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Cas7-11_T2A_Csx29

Expression

Mammalian

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T1

Plasmid

#201764

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/Lux-nat

Plasmid

#120324

Purpose

Bacterial luminescence

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Expression

Bacterial

Promoter

proD

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GssmCherry-PNE

Plasmid

#162157

Purpose

Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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HDR-HaloPOLQ

Plasmid

#211637

Purpose

halo donor

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

Puro-LoxHaloTag

Tags

flag

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet1

Plasmid

#218360

Purpose

Empty backbone with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMX222

Plasmid

#23109

Depositor

Linda Wordeman

Article

Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Mutation

S92E, S106E, S108E, S112E, S186E

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pLO87

Plasmid

#91726

Purpose

mycobacterial expression of mCherry from Hsp60 promoter

Depositor

Graham Hatfull

Article

Oldfield et al J Bacteriol. 2014 Oct;196(20):3589-97. d

Insert

hsp60 promoter

Use

Mycobacterial expression

Tags

mCherry

Promoter

hsp60

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pzntA_LacZ

Plasmid

#216325

Purpose

PZntA promoter driving expression of lacZ

Depositor

Mark Styczynski

Article

McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

Insert

b-Galactosidase

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pT7_zntR

Plasmid

#216324

Purpose

T7 promoter driving expression of zntR

Depositor

Mark Styczynski

Article

McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

Insert

Zinc-responsive activator

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKEW302-AcrVA4

Plasmid

#115656

Purpose

Expresses AcrVA4 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2G_2

Plasmid

#118225

Purpose

Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFF745

Plasmid

#61450

Purpose

IPTG-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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p7270 MSCV-N-HA-IRES-PURO Empty

Plasmid

#163302

Purpose

Empty vector control

Depositor

Peter Howley, Elizabeth White

Article

White et al J Virol. 2014 Aug;88(15):8201-12. doi: 1

Type

Empty backbone

Use

Retroviral

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pABY-T3

Plasmid

#201766

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPhlF-P3

Plasmid

#74687

Purpose

PhlF-P3 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSP3-PhlF-PPhlF-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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