We narrowed to 60,200 results for: cloning
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Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-luc2
Plasmid#138355PurposeGateway compatible middle entry clone contains Firefly Luciferase2 without minimal promoterDepositorInsertluciferase
ExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAMP-CY1_NF1 T1 (+minintr, KDR)
Plasmid#119200Purposetransfer vectorDepositorInsertNF1 type 1 mini-gene (NF1 Human)
UseTransfer vectorAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Puro_zhang2.0
Plasmid#167919PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
GGE146
Plasmid#120742PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_4UASxpr-TEF)DepositorInsertP1_4UASxpr-TEF
UseCloning/assembly plasmidAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA iRFP713
Plasmid#167908PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH71431
Plasmid#50344DepositorInsert3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-TurboID-V5-GSK3B_IDG-K
Plasmid#158164PurposeGateway destination clone of GSK3B (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-GSK3B (GSK3B Human)
UseLentiviral; Gateway destinationTagsTurboID-V5ExpressionMammalianPromoterUbiquitinAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_US71_APikL1
Plasmid#123646PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertUS71_APikL1
UseGolden gateAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRATIO3267
Plasmid#141312PurposeGateway cloning compatible dual bioluminescent ratiometric binary vectorDepositorTypeEmpty backboneTagsgLUC and redLUCExpressionPlantPromoterCaMV 35S promoterAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX4-AS-long (pcDNA3.1+)
Plasmid#75211PurposeMammalian expression of SLC24A4DepositorInsertSLC24A4 (SLC24A4 Human)
ExpressionMammalianMutationalternatively spliced exon presentPromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRATIO3212
Plasmid#141311PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneTagsmScarlet-I and mVenusExpressionPlantPromoterCaMV 35S promoterAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPOM033_P7_3"His5
Plasmid#216458Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''his5
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM026_P2_pNMT1
Plasmid#216451PurposeRegulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter nmt1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM019_P2_pRPL2102
Plasmid#216444PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rpl2102
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM015_P2_pADH1
Plasmid#216440PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter adh1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM013_P2_pENO101
Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGG010
Plasmid#113984PurposeVector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertRK2 (IncP)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB2a
Plasmid#19497DepositorInsertDNAJB2a
TagsGFPExpressionMammalianAvailable SinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
GGE367
Plasmid#120736PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_hph)DepositorInsertMarker_hph
UseCloning/assembly plasmidAvailable SinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only