We narrowed to 62,158 results for: cloning
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Plasmid#164583PurposeA mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.DepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
ExpressionMammalianMutationDeleted amino acids 1256-1273PromoterEF1aAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha Wildtype
Plasmid#163365PurposeExpress HA- and Clover-tagged HIF-1alpha wildtype plasmidDepositorInsertHypoxia Inducible Factor 1 alpha (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationWildtypePromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mito-GFP-ER tethering
Plasmid#160509PurposeRescue mfn2 knockdown by tethering mitochondria and ER with GFPDepositorInsertmito-GFP-ER tethering
UseLentiviralPromoterCMVAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPS-MYC
Plasmid#160130PurposeA reporter that monitors MYC protein expression.DepositorAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GAL4-DrBphP
Plasmid#158986PurposeExpresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cellsDepositorInsertGAL4-DrBphP
UseLentiviralTagsMyc tagExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-P2A-EGFP_mRFPstuf
Plasmid#137730PurposeExpresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNADepositorTypeEmpty backboneUseCRISPR, Lentiviral, Mouse Targeting, and Syntheti…ExpressionBacterial and MammalianAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA
Plasmid#156447PurposeFor transient expression of mouse SMC3 tagged with mKate2DepositorAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA
Plasmid#156436PurposeFor transient expression of human PDS5B tagged with mKate2DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Puro-Ak-TMPRSS2
Plasmid#154986PurposeLentiviral vector expressing Human TMPRSS2DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp8
Plasmid#154758PurposeExpression of SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialPromoterT7Available SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1397 DddAtox C
Plasmid#158097Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI
ExpressionMammalianMutationWTAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-E29-ChR2GFP2x
Plasmid#153437PurposeAAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E29 regulatory elementDepositorInsertChr2-GFP-P2A-GFP
UseAAVAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-ACE2-puro
Plasmid#158448PurposeConstitutive lentiviral expression of human ACE2.DepositorAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-ACE2-blast
Plasmid#158449PurposeConstitutive lentiviral expression of human ACE2.DepositorAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1397 DddAtox N
Plasmid#158094Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI
ExpressionMammalianMutationWTAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag
Plasmid#132399Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0
Plasmid#153291PurposeExpress the adenosine sensor GRAB_Ado1.0 in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterEFSAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC
Plasmid#126218PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseLentiviralExpressionMammalianPromoterCamKIIaAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-LSD1-Hygro
Plasmid#104406Purposetransient expression of dCas9-LSD1 fusion proteinDepositorInsertLSD1 (KDM1A Human)
UseCRISPRExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only