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Showing: 5541 - 5560 of 16055 results

  2. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid
    #117319
    Purpose
    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron
    Depositor
    Volker Busskamp
    Article
    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(
    Insert
    hsa-miR-CTRL
    Use
    Transposon-mediated integration
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    miR-124 deleted
    Promoter
    Tet-inducible promoter
    Available Since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    117319-plasmid-map-sequence-id-231738

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  3. UCP3 promoter EP1

    Plasmid
    #71743
    Purpose
    Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity
    Depositor
    Heinrich Taegtmeyer
    Article
    Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.
    Insert
    Uncoupling Protein 3 promoter (Ucp3 Mouse)
    Tags
    Luciferase
    Expression
    Bacterial
    Mutation
    Contains 7kb of the murine UCP3 gene encompassing…
    Promoter
    UCP3
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71743-plasmid-map-sequence-id-130165

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  4. pCDH-EMTB-TurboID-V5-puro

    Plasmid
    #190741
    Purpose
    A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
    Depositor
    Jeremy Baskin
    Article
    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021
    Insert
    EMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Microtubule binding-domain of ensconsin (amino ac…
    Promoter
    CMV
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    190741-plasmid-map-sequence-id-383769

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  5. pD649-HAsp-TNFRSF10B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156546
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TNFRSF10B (TNFRSF10B Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156546-plasmid-map-sequence-id-368521

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  6. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

    Plasmid
    #181851
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Lysine 128 in the DnaJB1-PKAcat fusion changed to…
    Promoter
    CMV
    Available Since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181851-plasmid-map-sequence-id-364311

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  7. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  8. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  9. MADR pDonor smFP-HA (dark) WPRE

    Plasmid
    #131007
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-HA WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    HA - 10 total HA epitope tages
    Expression
    Mammalian
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131007-plasmid-map-sequence-id-305185

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  10. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid
    #113961
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113961-plasmid-map-sequence-id-215291

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  12. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid
    #210710
    Purpose
    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9 (STAT1, MRTFA Staphylococcus aureus, Synthetic, Human)
    Use
    AAV and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/N580A
    Promoter
    M11
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210710-plasmid-map-sequence-id-416264

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  13. pD649-HAsp-EPCAM-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156693
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    EPCAM (EPCAM Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156693-plasmid-map-sequence-id-417861

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  14. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  17. pENT-L1-Kozak-EGFP-GPI-stop-L2

    Plasmid
    #186355
    Purpose
    Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Synthetic, Human)
    Use
    Expression of a fluorescent membrane marker
    Tags
    EGFP
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186355-plasmid-map-sequence-id-373423

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  18. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169180
    Purpose
    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6 (internal tag linking nsp7 and nsp8): nsp7-H…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169180-plasmid-map-sequence-id-333998

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  19. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  20. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169183
    Purpose
    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag (nsp12 C-terminus) and His6 (as internal t…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169183-plasmid-map-sequence-id-333983

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Showing: 5541 - 5560 of 16055 results