We narrowed to 30,619 results for: ica

1. pCm C-term MBP NikJC199U

   Plasmid

   #174365

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Use

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mito-ABKAR

   Plasmid

   #61509

   Purpose

   Expression of AMPK biosensor targeted to the mitochondria

   Depositor

   Jin Zhang, Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   AMPK Biosensor
   

   Use

   Tags

   Mitochondrial targeting signal (DAKAP1-30)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pGBT9-NF1-GRD

   Plasmid

   #19993

   Depositor

   Fuyuhiko Tamanoi

   Article

   Morcos et al Mol Cell Biol. 1996 May . 16(5):2496-503

   Insert

   Neurofibromatosis type I (NF1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-LOV-VP16

   Plasmid

   #42499

   Purpose

   LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   FKF1 (N-LOV, G128D mutant) fused with VP16
   

   Use

   Tags

   F-type VP16 activation domain and HA tag

   Expression

   Mammalian

   Mutation

   G128D 1-168aa

   Promoter

   CMV

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pShuttle-HA-FLAG-TIN2

   Plasmid

   #35025

   Depositor

   Sheila Stewart

   Article

   Nittis et al Mol Cell Proteomics. 2010 Jun;9(6):1144-

   Insert

   TIN2 (TINF2 Human)
   

   Use

   Adenoviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pHiUGE HA-epitope donor ORF1

   Plasmid

   #200372

   Purpose

   Tagging endogenously expressed proteins with HA epitope, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   HA epitope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXCas9H840A

   Plasmid

   #60900

   Purpose

   Dual Expression Vector for Cas9 H840A nickase and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL6

   Plasmid

   #48746

   Purpose

   Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region (-1128 to +2129)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pT2-cryR;cldn5bP1Egfp

   Plasmid

   #90158

   Purpose

   contains cldn5b specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   cldn5bP1Egfp (cldn5b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pH6HTC_IRP1-Halo

    Plasmid

    #175305

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IRP1 (ACO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKS17

    Plasmid

    #24628

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    pdc, adhB, atfA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBlu/gRNA

    Plasmid

    #59188

    Purpose

    Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Guide RNA Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6 (Arabidopis)

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDA056

    Plasmid

    #131387

    Purpose

    ORFeus expression, WT

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus-Hs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX-EF1a_NKX2-1_Short_V5-GFP

    Plasmid

    #221497

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)

    Plasmid

    #104119

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    CheRiff-CFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ki67∆FHA-mNeonGreen-IRESpuro2

    Plasmid

    #183739

    Purpose

    Expresses Ki67∆FHA-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67_lacking FHA (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67(lacking FHA)

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBac{3XP3::EYFP,attP}

    Plasmid

    #86860

    Purpose

    Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    attP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF3BGX-T2A-GAL4-DBD

    Plasmid

    #138394

    Purpose

    RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL4-DBD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUB_smFLAG_ActB_MS2

    Plasmid

    #81083

    Purpose

    Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

    Depositor

    Tim Stasevich

    Article

    Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

    Insert

    smFLAG_ActB_24xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p5E-EF1a/b-actin

    Plasmid

    #82583

    Purpose

    5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCIP-caTfeb

    Plasmid

    #79013

    Purpose

    For mammalian expression of a shortened, constitutively active version of Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Tfeb (TFEB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removed first ~400 bp from 5' end (N-terminu…

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pGFAP-TetOn3G

    Plasmid

    #133728

    Purpose

    Plasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    TetOn3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGFAP

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SpyCatcher002-oDi

    Plasmid

    #124661

    Purpose

    Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oDi
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163072

    Purpose

    Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-His-SCov2-nsp7

    Plasmid

    #154757

    Purpose

    Expression of SARS-CoV-2 nsp7

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pH6HTC_FAM98A-Halo

    Plasmid

    #175329

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    FAM98A (FAM98A Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMM289

    Plasmid

    #46037

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN E84A/K577M (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A/K577M exonuclease and helicase-dead mutant; …

    Promoter

    CMV2

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJJB1561

    Plasmid

    #218626

    Purpose

    Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMM287

    Plasmid

    #46035

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN K577M (WRN Human)
    

    Use

    Teton, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    K577M helicase-dead mutant; S2G introduced during…

    Promoter

    CMV2

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-UBC9-K14R-A129K

    Plasmid

    #228948

    Purpose

    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    changed Lysine 14 to Arginine, changed Alanine 12…

    Promoter

    T7-lac

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag-Daxx/pRK5

    Plasmid

    #27974

    Depositor

    Xiaolu Yang

    Article

    Tang et al Nat Cell Biol. 2006 Aug . 8(8):855-62.

    Insert

    Daxx (DAXX Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pShuttle-FEN1hWT

    Plasmid

    #35027

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1 (FEN1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

36. DNA-PKcs N-terminal sgRNA

    Plasmid

    #207093

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGCGGGACTCGGCGGCATGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Halo-DNAPKcs HRD

    Plasmid

    #207088

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTP341 pHAGE2 CMV TagBFP-3xFLAG

    Plasmid

    #199391

    Purpose

    Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    TagBFP-3xFLAG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. actc1b-Lifeact-mKate2

    Plasmid

    #109493

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequence

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-Lifeact-mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. His6_MBP_FXR2P_P474S_P493N

    Plasmid

    #198695

    Purpose

    Expression of human FXR2 protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FXR2P (FXR2 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Changed proline 474 to serine and proline 493 asp…

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge