We narrowed to 9,361 results for: Pol;

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pDT386

Plasmid

#174733

Purpose

Expresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.

Depositor

Jukka Finne

Article

Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

Insert

DT386

Tags

Histag

Expression

Bacterial

Mutation

Deleted amino acids 412-560 (the receptor binding…

Promoter

T5

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mycRSK2wt

Plasmid

#15827

Depositor

Jing Chen

Article

Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

Insert

Ribosomal S6 kinase 2 (Rps6ka3 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

wild type

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-Tagger

Plasmid

#129396

Purpose

Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

Insert

rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2

Tags

FLAG, HA, and V5

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA_Tet3

Plasmid

#85740

Purpose

shRNA against mouse Tet3

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

Tet3 (Tet3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-OF (mutant TCF-4 sites)

Plasmid

#16559

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

TCF-4 binding sites (TCF4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

3 copies of mutant Tcf-4 binding sites

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM4-Flag

Plasmid

#180409

Purpose

lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM4 (TGM4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM5-V5

Plasmid

#180414

Purpose

lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM5 (TGM5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mIL-2Rα

Plasmid

#59917

Purpose

Expresses mouse interleukin-2 receptor alpha chain

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-SYN-L-sDarken

Plasmid

#184798

Purpose

Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

L-sDarken

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGM8

Plasmid

#220166

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TSPO (TSPO Human)

Use

Lentiviral

Tags

GFP11

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX Flag RagC S75N

Plasmid

#112757

Purpose

express Flag-RagC (S75N mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (S75N) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Mutation

S75N

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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cyclin D1-HA-pBabe-Hygro

Plasmid

#22703

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

cyclin D1 (CCND1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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LLP109 pEF1a-gRNA-GFP

Plasmid

#100935

Purpose

GFP, puromycin and gRNA scaffold driven by EF1a promoter

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

gRNA scaffold

Use

Grna scaffold

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pNG1256

Plasmid

#149710

Purpose

Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

rpoZ (rpoZ )

Expression

Bacterial

Promoter

T7

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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CIBN-GFP-TMD

Plasmid

#79571

Purpose

expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

syntaxin 1A (Stx1a Mouse)

Tags

CIBN and EGFP

Expression

Mammalian

Mutation

aa 249_288 (C-terminal region (polybasic stretch …

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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hsp70: mKate2-DN Kif5a

Plasmid

#105972

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Tags

mKate2

Expression

Bacterial

Mutation

truncation of aa416-1033

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTon1(acgfp1)

Plasmid

#233648

Purpose

Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

Depositor

Takeo Kubo

Article

Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

Insert

target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'

Use

Amphibian expression, tet-on

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG32-3HA-3FLAG

Plasmid

#154081

Purpose

For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

Depositor

Cornelia Kilchert

Article

Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

Insert

3xHA-3xFLAG-hygR

Use

Pcr-based tagging in yeast

Tags

3xHA-3xFLAG

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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-227 hTF (mutant AP-1)

Plasmid

#15444

Depositor

Nigel Mackman

Article

Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Both AP-1 sites mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only