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Plasmid#187724PurposeExpress His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cellsDepositorInsertNEDD4L residues 153-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 1-152 and 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (381-975)_pcDNA3.1(+)
Plasmid#187725PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-380PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (492-975)_pcDNA3.1(+)
Plasmid#187726PurposeExpress his-tagged NEDD4L W3-W4-HECT in mammalian cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-491PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)
Plasmid#187727PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 226-382PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)
Plasmid#187728PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 418-495PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT_C874only
Plasmid#187711PurposeExpress his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cellsDepositorInsertNEDD4L residues 492-975 with mutations (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491; mutations C702A, C776…PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_Δ1,2-linker
Plasmid#187714PurposeExpress GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 226-382PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_Δ2,3-linker
Plasmid#187715PurposeExpress GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 418-495PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-HHEX
Plasmid#222928PurposePiggyBac transposon plasmid for doxycycline inducible expression of HHEXDepositorInsertHHEX (HHEX Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 mOct4-POU
Plasmid#206393PurposeExpresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purificationDepositorInsertOct4 (Pou5f1 Mouse)
UseTags6xHisExpressionBacterialMutationPOU DNA binding domain onlyPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_494-546
Plasmid#196531PurposeEntry vector for Gateway with Eps8L2_494-546DepositorInsertEps8L2_494-546 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_367-496
Plasmid#196530PurposeEntry vector for Gateway with Eps8L2_367-496DepositorInsertEps8L2_367-496 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_1-183
Plasmid#196527PurposeEntry vector for Gateway with Eps8L2_1-183DepositorInsertEps8L2_1-183 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_544-715
Plasmid#196532PurposeEntry vector for Gateway with Eps8L2_544-715DepositorInsertEps8L2_544-715 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_181-299
Plasmid#196528PurposeEntry vector for Gateway with Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-Eps8L2_1-496
Plasmid#196516PurposeExpression of GST-Eps8L2_1-496DepositorInsertEps8L2_1-496 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationSilent mutations for siRNA resistancePromoterlac promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_1-183
Plasmid#196535PurposeExpression of GST-Eps8L2_1-183DepositorInsertEps8L2_1-183 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_181-299
Plasmid#196536PurposeExpression of GST-Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_544-715
Plasmid#196540PurposeExpression of GST-Eps8L2_544-715DepositorInsertEps8L2_544-715 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nmyc-Eps8L2-FL-siR
Plasmid#196514PurposeExpression of Myc-Eps8L2-FLDepositorInsertEps8L2-FL-siR (EPS8L2 Human)
UseTagsMycExpressionMammalianMutationSilent mutations for siRNA resistancePromoterT7 promoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_529-715
Plasmid#196533PurposeEntry vector for Gateway with Eps8L2_529-715DepositorInsertEps8L2_529-715 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_297-370
Plasmid#196537PurposeExpression of GST-Eps8L2_297-370DepositorInsertEps8L2_297-370 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_367-496
Plasmid#196538PurposeExpression of GST-Eps8L2_367-496DepositorInsertEps8L2_367-496 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_494-546
Plasmid#196539PurposeExpression of GST-Eps8L2_494-546DepositorInsertEps8L2_494-546 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS97splitRBP_S1dC2
Plasmid#183769PurposeExpresses SHIP1-delta-C2-Enzyme domain flanked by RBPDepositorInsertSHIP1deltaC2 (INPP5D Human)
UseTags6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…ExpressionBacterialMutationSHIP1deltaC2 includes aa N393 to aa Q730 onlyPromoterT7lacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP1
Plasmid#183770PurposeExpresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP1 (INPP5D Human)
UseTags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa1 to aa883 onlyPromoterT7 lacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS97splitRBP_S1-Enz
Plasmid#183767PurposeExpresses SHIP1-Enzyme domain flanked by RBPDepositorInsertSHIP1 (INPP5D Human)
UseTags6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…ExpressionBacterialMutationincludes aa N393 to aa L864 onlyPromoterT7lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP2
Plasmid#183771PurposeExpresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP2 (INPPL1 Human)
UseTags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa18 to aa985 onlyPromoterT7 lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKHH030_sgCtrl#2
Plasmid#174143PurposeLentiviral vector expressing a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plxnb2(S)-AP-His
Plasmid#72003PurposeExpresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb2 (Plxnb2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only