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Showing: 1081 - 1120 of 1308 results

  1. pOPO717

    Plasmid
    #195302
    Purpose
    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and lacZ spacer 5 in native array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195302-plasmid-map-sequence-id-383138

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  2. pOPO812

    Plasmid
    #195306
    Purpose
    pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195306-plasmid-map-sequence-id-383143

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  3. pOPO646

    Plasmid
    #195307
    Purpose
    pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    TnsD of McCAST
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195307-plasmid-map-sequence-id-383139

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  4. pOPO685

    Plasmid
    #195300
    Purpose
    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and native array with PaqCI entry site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195300-plasmid-map-sequence-id-394739

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  5. pOPO794

    Plasmid
    #195303
    Purpose
    pBAD322-MycaIDCas-ΔCas6-RGR-PaqCI, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked PaqCI entry site.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade Δcas6 and ribozyme flanked entry site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195303-plasmid-map-sequence-id-383144

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  7. pTV001

    Plasmid
    #130265
    Purpose
    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA::His8
    Use
    Tags
    polyhistidine (internal)
    Expression
    Bacterial
    Mutation
    8His polyhistidine encoding region and XhoI site …
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    130265-plasmid-map-sequence-id-257613

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  9. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  12. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA-DUF::His8
    Use
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    129732-plasmid-map-sequence-id-257612

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  18. pCAM9

    Plasmid
    #78560
    Purpose
    pBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.
    Depositor
    James Kaper
    Article
    Merrell et al J Bacteriol. 2001 May;183(9):2746-54.
    Insert
    ompU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  22. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Use
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

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  23. pH6-TEV-spTorA-GFP(C)

    Plasmid
    #169041
    Purpose
    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    H6-spTorA-GFP
    Use
    Tags
    6xHis, TEV cleavage site, and spTorA (signal pept…
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169041-plasmid-map-sequence-id-346121

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  24. pZH_tdPP7cp_mCherry

    Plasmid
    #118267
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCherry
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118267-plasmid-map-sequence-id-229509

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  25. pZH_mPP7cp_mCerulean3

    Plasmid
    #118262
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCerulean3
    Depositor
    Zach Hensel
    Insert
    mPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118262-plasmid-map-sequence-id-229514

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  26. pZH_tdPP7cp_mCerulean3

    Plasmid
    #118263
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCerulean3
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118263-plasmid-map-sequence-id-229501

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  27. pZH_mPP7cp_mCherry

    Plasmid
    #118266
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCherry
    Depositor
    Zach Hensel
    Insert
    mPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118266-plasmid-map-sequence-id-229515

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  28. pZH_tdPP7cp_ECFP

    Plasmid
    #118265
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-ECFP
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118265-plasmid-map-sequence-id-229511

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  29. pZH_mPP7cp_ECFP

    Plasmid
    #118264
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-ECFP
    Depositor
    Zach Hensel
    Insert
    mPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118264-plasmid-map-sequence-id-229507

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  30. pKP1912

    Plasmid
    #167600
    Purpose
    Express ExbB delta(235-244),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 235-24…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167600-plasmid-map-sequence-id-339838

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  31. pKP2040

    Plasmid
    #167650
    Purpose
    Express His6-ExbB S80A, S82A, S84A
    Depositor
    Kathleen Postle
    Insert
    his6-exbB S80A, S82A, S84A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ExbB S80A, S82A, S84A with His6 tag at N terminus
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167650-plasmid-map-sequence-id-337301

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  32. pKP1940

    Plasmid
    #167599
    Purpose
    Express ExbB delta(225-234),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 225-23…
    Promoter
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    167599-plasmid-map-sequence-id-333697

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  33. pKP1965

    Plasmid
    #167598
    Purpose
    Express ExbB delta(215-224),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 215-22…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167598-plasmid-map-sequence-id-332908

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  34. pKP1964

    Plasmid
    #167597
    Purpose
    Express ExbB delta(205-214),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 205-21…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167597-plasmid-map-sequence-id-332907

    Enlarge

  35. pKP1963

    Plasmid
    #167596
    Purpose
    Express ExbB delta(195-204),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 195-20…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167596-plasmid-map-sequence-id-332906

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  38. pKP2009

    Plasmid
    #167602
    Purpose
    Express ExbB (215-224 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 215-22…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167602-plasmid-map-sequence-id-339839

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  39. pKP2010

    Plasmid
    #167603
    Purpose
    Express ExbB (235-244 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 235-24…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167603-plasmid-map-sequence-id-339840

    Enlarge

  40. pKP2019

    Plasmid
    #167604
    Purpose
    Express ExbB (195-204 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 195-20…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167604-plasmid-map-sequence-id-333698

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Showing: 1081 - 1120 of 1308 results