We narrowed to 1,445 results for: pBAD
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Plasmid#155154PurposeExpresses MlaF(1-246)-(14 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0.093
Plasmid#119574PurposeLevel 0 part. PromoterDepositorInsertPBAD
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2m
Plasmid#105066PurposeA yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cellsDepositorInsertY-GECO2m
Tags6x His tagExpressionBacterialPromoterpBADAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVH004
Plasmid#80401PurposeContains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.DepositorUseSynthetic BiologyTagsC-terminal LZx domainExpressionBacterialAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHYBAD94
Plasmid#42305DepositorInsertNpuDnaEC6
TagsGB1-H6ExpressionBacterialPromoteraraBADAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
Tags6x His tagExpressionBacterialPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
Tags6x His tagExpressionBacterialPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRExpressionBacterialPromoterpBADAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
TagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialPromoterpDABAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterP(rhaB)Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickExpressionBacterialPromoterPbadAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15ABAD- YFP
Plasmid#186571Purposep15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterBAD promoterAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWJB9
Plasmid#126650PurposeExpresses AdrA from a modified pLAFR3DepositorInsertadrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
Tags8xHisExpressionBacterialPromoterPBADAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
ExpressionBacterialPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: pBAD, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-MESVD/TRAP(RK)-C
Plasmid#61967PurposeDuet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVDDepositorInsertsN-mCherry-MESVD
TRAP(RK)-C-mCherry
ExpressionBacterialPromoterPBAD and PLtetOAvailable SinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7RNAPNev-NB1
Plasmid#216336PurposeArabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1DepositorInsertT7RNAPN-NB1 fusion
ExpressionBacterialAvailable SinceJuly 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLN34 - pBait-5GC[1xMS2]-McaS
Plasmid#222403Purposearabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAMC042z21
Plasmid#209747PurposeRepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL blaDepositorInsertsfGFP - tRNA operon
ExpressionBacterialMutationsfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- BAD-YFP
Plasmid#186567PurposepMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterBAD promoterAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1936
Plasmid#195692PurposeComplementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaBDepositorInsertMlaFEDCB
ExpressionBacterialMutationMlaF-MlaE-MlaDdelta153-183-MlaC-MlaBAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1935
Plasmid#195693PurposeComplementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaBDepositorInsertMlaFEDCB
ExpressionBacterialMutationMlaF-MlaE-MlaDdelta173-183-MlaC-MlaBAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1953
Plasmid#195694PurposeComplementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaBDepositorInsertMlaFEDCB
ExpressionBacterialMutationMlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaBAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1933
Plasmid#195695PurposeComplementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaBDepositorInsertMlaFEDCB
ExpressionBacterialMutationMlaF-MlaE-MlaD(F118A)-MlaC-MlaBAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1934
Plasmid#195696PurposeComplementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaBDepositorInsertMlaFEDCB
ExpressionBacterialMutationMlaF-MlaE-MlaD(L123A)-MlaC-MlaBAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2766
Plasmid#196028PurposeComplementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaBDepositorInsertMlaFEDCB
ExpressionBacterialMutationMlaF-MlaE-MlaDdelta141-183-MlaC-MlaBAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBXPHM3-NB-D6
Plasmid#167989Purposeexpression of a nanobody against rat PepT2 as a n terminal MBP fusionDepositorInsertNanobodyD6
TagsHis-MBPExpressionBacterialPromoterpBADAvailable SinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2196
Plasmid#170400PurposeFor expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-717, replaced with 114-130)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2197
Plasmid#170401PurposeFor expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 347-360)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2198
Plasmid#170402PurposeFor expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 580-600)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2251
Plasmid#170403PurposeFor expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 225-249)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2252
Plasmid#170404PurposeFor expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-716, replace with 455-479)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2253
Plasmid#170405PurposeFor expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 812-836)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+7U
Plasmid#149393PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation7bp inserted upstream, and 10bp inserted downstre…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+10U
Plasmid#149394PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation10bp inserted upstream and downstream of the IHFp…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB201
Plasmid#169112PurposeTESEC expression vector for Alr from M. tuberculosisDepositorInsertalr (alr Mycobacterium tuberculosis H37Rv)
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only