Skip to main content
Addgene
Showing: 1351 - 1400 of 2419 results

  6. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118163-plasmid-map-sequence-id-240400

    Enlarge

  8. pHAGE pGK ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

    Plasmid
    #215013
    Purpose
    Express a ERphagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    KDEL
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215013-plasmid-map-sequence-id-429268

    Enlarge

  12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide4

    Plasmid
    #125459
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125459-plasmid-map-sequence-id-251390

    Enlarge

  13. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide3

    Plasmid
    #125458
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125458-plasmid-map-sequence-id-251380

    Enlarge

  14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide2

    Plasmid
    #125457
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125457-plasmid-map-sequence-id-251379

    Enlarge

  15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide1

    Plasmid
    #125456
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125456-plasmid-map-sequence-id-251378

    Enlarge

  37. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide1

    Plasmid
    #125393
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    125393-plasmid-map-sequence-id-251084

    Enlarge

  39. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118164
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118164-plasmid-map-sequence-id-240433

    Enlarge

  40. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide2

    Plasmid
    #118201
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118201-plasmid-map-sequence-id-240397

    Enlarge

  42. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide4

    Plasmid
    #118203
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118203-plasmid-map-sequence-id-240387

    Enlarge

  43. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

    Plasmid
    #118202
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118202-plasmid-map-sequence-id-240385

    Enlarge

Showing: 1351 - 1400 of 2419 results