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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,346 results for: rel

Showing: 1351 - 1400 of 6346 results

  4. CMV-mito-R-GECO1

    Plasmid
    #46021
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    46021-plasmid-map-sequence-id-350822

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  6. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Use
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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  7. pDN-D2irTNG4kwh

    Plasmid
    #44722
    Depositor
    Gabor Balazsi
    Article
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Inserts
    pCMV-D2i promoter
    htetR::NLS::EGFP
    Use
    Synthetic Biology; Expression regulator/reporter;…
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44722-plasmid-map-sequence-id-61263

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  8. LSB-hsa-miR-92a-1-5p

    Plasmid
    #103750
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-92a-1-5p target (MIR92A1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103750-plasmid-map-sequence-id-196548

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  9. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

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  10. pUC57-BBclone1

    Plasmid
    #137069
    Purpose
    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Inserts
    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    Use
    Tags
    Expression
    Bacterial
    Mutation
    wild-type, full-length (signal-peptide-containing…
    Promoter
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137069-plasmid-map-sequence-id-275695

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  11. hIgG1_heavy_CoVMab7

    Plasmid
    #194605
    Purpose
    Mammalian expression of CoVMab7 heavy chain
    Depositor
    Ben Murrell
    Insert
    CoVMab7 heavy chain (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194605-plasmid-map-sequence-id-381536

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  12. hIgG1_heavy_CoVMab4

    Plasmid
    #194603
    Purpose
    Mammalian expression of CoVMab4 heavy chain
    Depositor
    Ben Murrell
    Insert
    CoVMab4 heavy chain (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194603-plasmid-map-sequence-id-381534

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  13. hIgG1_kappa_CoVMab7

    Plasmid
    #194606
    Purpose
    Mammalian expression of CoVMab7 light chain
    Depositor
    Ben Murrell
    Insert
    CoVMab7 light chain (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194606-plasmid-map-sequence-id-381532

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  14. hIgG1_kappa_CoVMab4

    Plasmid
    #194604
    Purpose
    Mammalian expression of CoVMab4 light chain
    Depositor
    Ben Murrell
    Insert
    CoVMab4 light chain (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194604-plasmid-map-sequence-id-381530

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  15. pKPY738

    Plasmid
    #72674
    Purpose
    C. elegans let-858 3' UTR (Gateway Entry Clone)
    Depositor
    David Tirrell
    Insert
    let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72674-plasmid-map-sequence-id-133995

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  16. pKPY115

    Plasmid
    #72669
    Purpose
    Encodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control
    Depositor
    David Tirrell
    Insert
    Thr251Gly-EcPheRS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Thr251Gly in Alpha Subunit
    Promoter
    PBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72669-plasmid-map-sequence-id-133221

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  17. hIgG1-F1-Fc

    Plasmid
    #194597
    Purpose
    Mammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    F1-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194597-plasmid-map-sequence-id-382112

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  18. hIgG1-E2-Fc

    Plasmid
    #194595
    Purpose
    Mammalian expression of E2 nanobody upstream of human IgG1; E2 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    E2-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194595-plasmid-map-sequence-id-381544

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  19. hIgG1-G6-Fc

    Plasmid
    #194594
    Purpose
    Mammalian expression of G6 nanobody upstream of human IgG1; G6 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    G6-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194594-plasmid-map-sequence-id-381539

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  20. hIgG1-C7-Fc

    Plasmid
    #194596
    Purpose
    Mammalian expression of C7 nanobody upstream of human IgG1; C7 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    C7-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194596-plasmid-map-sequence-id-381540

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  23. pKPY737

    Plasmid
    #72673
    Purpose
    Encodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)
    Depositor
    David Tirrell
    Insert
    Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Thr412Gly in CePheRS Alpha Subunit
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72673-plasmid-map-sequence-id-133998

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  39. pcDNA216-CoV2-RBD-gamma variant

    Plasmid
    #194600
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 gamma
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-gamma variant (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194600-plasmid-map-sequence-id-381528

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  40. pcDNA216-CoV2-RBD-delta variant

    Plasmid
    #194601
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 delta
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-delta variant (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194601-plasmid-map-sequence-id-381523

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  41. pcDNA216-CoV2-RBD-alpha variant

    Plasmid
    #194599
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 alpha
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-alpha variant (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194599-plasmid-map-sequence-id-381522

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Showing: 1351 - 1400 of 6346 results