Addgene

Showing: 541 - 560 of 16703 results

Results per page:

Sort results by:

pAPInt-mCherry-Hyg

Plasmid

#236489

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mCherry

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-GFP-Nat

Plasmid

#236476

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-Dendra2-Hyg

Plasmid

#236495

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

Dendra2

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mNeonGreen-Hyg

Plasmid

#236483

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mScarlet-Hyg

Plasmid

#236492

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mScarlet

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-GFP-Hyg

Plasmid

#236477

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-sfGFP-Nat

Plasmid

#236479

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mNeonGreen-Nat

Plasmid

#236482

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_886_Nhe2_EcR-to-ato_RB

Plasmid

#71181

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

886_Nhe2_EcR-to-ato_RB

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_975_Nhe2_EcR-mut

Plasmid

#71179

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

975_Nhe2_EcR-mut

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11_DHNTPase

Plasmid

#73154

Purpose

Expresses in E. coli, E. coli Dihydroneopterin triphosphate pyrophosphatase

Depositor

Maurice Bessman, Sandra Gabelli

Article

Gabelli et al Structure. 2007 Aug;15(8):1014-22.

Insert

ORF17_DHNTPase_NUD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2016

Availability

Academic Institutions and Nonprofits only
pJK500

Plasmid

#72245

Purpose

Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

T. J. Kappock

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

Expression

Bacterial

Mutation

Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

Promoter

T7 and endogenous

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK392

Plasmid

#70236

Purpose

Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40N

Depositor

T. J. Kappock

Article

Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

Insert

phosphoribosylaminoimidazole carboxylase

Use

Tags

Expression

Bacterial

Mutation

His40Asn

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_711_OSC-fkh_dCP

Plasmid

#71220

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

711_OSC-fkh_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_871_Nhe2_EcR-to-ato

Plasmid

#71180

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

871_Nhe2_EcR-to-ato

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_889_Nhe2_EcR-to-Ets96B_RB

Plasmid

#71183

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

889_Nhe2_EcR-to-Ets96B_RB

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_881_Nhe2_EcR-to-HLH4C

Plasmid

#71186

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

881_Nhe2_EcR-to-HLH4C

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_900_Nhe2_EcR-to-gl_RB

Plasmid

#71185

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

900_Nhe2_EcR-to-gl_RB

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_892_Nhe2_EcR-to-HLH4C_RB

Plasmid

#71187

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

892_Nhe2_EcR-to-HLH4C_RB

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_575_Ubi-2-Trl_dCP

Plasmid

#71167

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

575_Ubi-2-Trl_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge