We narrowed to 6,639 results for: WUS

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pDA056

Plasmid

#131387

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Expression

Mammalian

Promoter

TRE3G

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124431

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Zebrafish (if expression level is too high in zeb…

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6

Plasmid

#121151

Purpose

Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K6 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132399

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDA083

Plasmid

#131389

Purpose

eGFP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

eGFP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA, mitfa:Cas9

Plasmid

#118844

Purpose

Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3G-BE3

Plasmid

#113415

Purpose

Expresses hA3G-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_HSP90B1_WT

Plasmid

#82130

Purpose

Gateway Donor vector containing HSP90B1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSP90B1 (HSP90B1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1

Plasmid

#218796

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_MDM2_WT

Plasmid

#82897

Purpose

Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MDM2 (MDM2 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT3_WT

Plasmid

#82235

Purpose

Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.L858R

Plasmid

#82906

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

L858R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pYC-C

Plasmid

#207114

Purpose

HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

Depositor

Yifan Cheng

Article

Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiGuide

Plasmid

#117986

Purpose

pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

Depositor

Paul Khavari

Article

Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

Insert

sgRNA backbone (F+E)

Use

Lentiviral

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_WT

Plasmid

#81736

Purpose

Gateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CA (PIK3CA Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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JWW-2 human chimeric monoclonal antibody

Plasmid

#66749

Purpose

Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-2 Heavy Chain
JWW-2 Light Chain

Expression

Mammalian

Promoter

mEF1 and rEF1

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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JWW-1 human chimeric antibody

Plasmid

#66748

Purpose

Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa

Expression

Mammalian

Mutation

First 3 amino acids of rat light chain constant r…

Promoter

mEF1 and rEF1

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pY31

Plasmid

#84746

Purpose

Expresses huLbCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATG16L1_WT

Plasmid

#82255

Purpose

Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATG16L1 (ATG16L1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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