We narrowed to 9,088 results for: ef1

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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryCXCR4-W

Plasmid

#200102

Purpose

Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR and Lentiviral

Mutation

silent mutations (c.180C>T, 438G>A, 495C>…

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NucGFP

Plasmid

#184706

Purpose

Lentiviral transfer plasmid for expression of NucGFP

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

NucGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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R777-E223 Hs.RAPGEF1

Plasmid

#70507

Purpose

Gateway ORF clone of human RAPGEF1 [NM_198679.1] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RAPGEF1 (RAPGEF1 Human)

Use

Gateway entry clone

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1a_CD MUT TET1

Plasmid

#124083

Purpose

Expression of mutated catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - catalytic domain mutated (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

H1672Y, D1674A

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-mutFAKSPARK2

Plasmid

#195266

Purpose

express Tyr to Phe mutant FAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

Tyr to Phe mutant FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1A-BRD3R

Plasmid

#130696

Purpose

Lentiviral plasmids encoding human BRD3R

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

Human BRD3R (BRD3 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Bamh1

Plasmid

#203308

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBS505 EF1alpha-EGFPcre*

Plasmid

#11955

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-Backbone(NN)

Plasmid

#27961

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NN)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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p413TEF1-ATR1(pYL573)

Plasmid

#178288

Purpose

Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

ATR1

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBS500 EF1alpha-GFPcre

Plasmid

#11920

Depositor

Brian Sauer

Article

Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
LV_EF1a_MAPK14-P2A-Hygro_Barcode

Plasmid

#170239

Purpose

Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK14 (MAPK14 Human)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-miRFP670nano3

Plasmid

#207747

Purpose

Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-miRFP670nano3

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-EF1a-SOX2

Plasmid

#35388

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Sox2

Use

Lentiviral

Expression

Mammalian

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pEF1alpha-mClover3-bGHpA

Plasmid

#172286

Purpose

Constitutive expression of mClover3

Depositor

Patrik Verstreken

Insert

mClover3

Expression

Bacterial

Promoter

EF1alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF16

Plasmid

#52642

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF16

Expression

Mammalian

Mutation

D431N/D433N/D435N/E442A mutated from GCaMP3

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only