We narrowed to 9,067 results for: sgRNA

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Publication

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Publication

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

Plasmid

#189924

Purpose

AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Publication

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

SaCas9 D10A, TadA ABE8e V106W

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR8-attL3-pegRNA-attL2

Plasmid

#213052

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA-msIM83

Plasmid

#251968

Purpose

sgRNA for yeast mtDNA editing, with IM83 aptamer inserted into the major stem-loop of the sgRNA

Depositor

Alice Ting

Publication

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

sgRNA-msIM83

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

Plasmid

#118154

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

EF1a core and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCR8-attL1-pegRNA-attR3

Plasmid

#213051

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Construct 9 - U6p_30nt_stgRNA

Plasmid

#81248

Purpose

Expresses 30nt stgRNA

Depositor

Timothy Lu

Publication

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

122 Articles

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pXPR_071

Plasmid

#164558

Purpose

Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

Depositor

Arlene Sharpe

Publication

LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

Insert

sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLCKO_PSMD1_sgRNA_5

Plasmid

#74181

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Publication

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC103

Plasmid

#62340

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHvL1P4GA

Plasmid

#112028

Purpose

Expresses sgRNA in barley

Depositor

Wendy Harwood

Publication

Lawrenson et al Methods Mol Biol. 2019;1900:217-232. doi

Insert

TaU6-LacZ-sgRNA

Use

Unspecified

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPSG804

Plasmid

#239534

Purpose

CRISPR plasmid with Cas9 and sgRNA targeting YDR514C locus in S. cerevisiae

Depositor

John Dueber

Insert

sgRNA_YDR514C

Use

CRISPR

Available since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSG779

Plasmid

#239532

Purpose

CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae

Depositor

John Dueber

Insert

sgRNA_YBR197C

Use

CRISPR

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPSG803

Plasmid

#239533

Purpose

CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae

Depositor

John Dueber

Insert

sgRNA_YMR206W

Use

CRISPR

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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