We narrowed to 9,184 results for: Pol;

1. pCS5

   Plasmid

   #55750

   Purpose

   Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 59-264 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-SmB'

   Plasmid

   #1842

   Depositor

   Philip Leder

   Article

   Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

   Insert

   SmB' (SNRPB Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pBTK550d

   Plasmid

   #110617

   Purpose

   BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pJet-CMV-CD5HA2-bglpA

   Plasmid

   #96962

   Purpose

   Expression plasmid for HA-tag embedded into human CD52 with short polyA

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CMV-CD5HA2-bglpA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TIA1 RRM-CFP-FRBx5

   Plasmid

   #103780

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   TIA1 RRM-CFP-FRBx5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC121

   Plasmid

   #118893

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC70

   Plasmid

   #118878

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, cleavable with TEV protease; HIS6, cleavabl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUWR-CadTS

   Plasmid

   #58325

   Purpose

   Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

   Depositor

   Denise Montell

   Article

   Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

   Insert

   E-cadherin tension sensor
   

   Expression

   Insect

   Promoter

   poly-ubiquitin

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pRJPaph-HcRed

   Plasmid

   #67035

   Purpose

   Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   HcRed
   

   Use

   Conjugative bacterial vector

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEC626

    Plasmid

    #174367

    Purpose

    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23106

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK RagA-RagC

    Plasmid

    #99649

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    10x His tag, Poly arginine linker, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. DPAGT1 (DPAGT1A-c005)

    Plasmid

    #98228

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DPAGT1A (DPAGT1 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    pfam00953:Glycos_transf_4 98-275

    Promoter

    polyhedrin

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC92

    Plasmid

    #118910

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Tags

    eGFP-HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC6

    Plasmid

    #118864

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, EGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD_OsU3 (GB1184)

    Plasmid

    #68209

    Purpose

    Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    OsU3 promoter
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPAtUbq10 (GB0223)

    Plasmid

    #68174

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq10 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

    Plasmid

    #162918

    Purpose

    Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC204

    Plasmid

    #118894

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mKate2, HIS6,…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC81

    Plasmid

    #118900

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GST

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease; GST tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEXP5-NT-CuSeCat

    Plasmid

    #42568

    Depositor

    Ivan Korendovych

    Article

    Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

    Insert

    mutated calmodulin gene
    

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Valin…

    Promoter

    T7

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175784

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSBL_DK198

    Plasmid

    #156425

    Purpose

    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    aa 42-275

    Promoter

    Polyhedrin

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUWR-Cad-mTFP

    Plasmid

    #58327

    Purpose

    Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-mTFP
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pOCC215

    Plasmid

    #118879

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    

    Tags

    Dendra2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLVTHM-shREV3-4

    Plasmid

    #38033

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

    Insert

    shREV3-4 (Rev3l, Rev3l, REV3L Human, Rat, human, Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pOCC207

    Plasmid

    #118869

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mCherry

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

    Plasmid

    #130665

    Purpose

    Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
    

    Use

    Ae. aegypti expression

    Expression

    Insect

    Promoter

    Aedes aegypti poly-ubiquitin

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCRII-Topo Nectin-3 in situ probe

    Plasmid

    #45633

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Nectin-3 in situ probe (Nectin3 Mouse)
    

    Use

    In situ

    Mutation

    fragment contains bp# 1626-2039 of NM_021495

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pOCC230

    Plasmid

    #118871

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mCitrine, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOCC69

    Plasmid

    #118874

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP, cleava…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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