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We narrowed to 18,759 results for: nls

Showing: 11201 - 11240 of 18759 results
  1. PX390 Flavobacterium columnare Cas9

    Plasmid
    #68327
    Purpose
    Flavobacterium columnare Cas9
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. CFP(159-238)-beta-1 in pcDNAI/Amp

    Plasmid
    #55592
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. CFP(159-238)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55777
    Purpose
    A C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP(159-238)-gamma-2 (GNG2 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. YFP(159-238)-gamma-7 in pcDNAI/Amp

    Plasmid
    #54473
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(159-238)-gamma-7 (GNG7 Human, Aequorea victoria)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-7 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pMuLE ENTR CMV-hCas9 R4-R3

    Plasmid
    #62132
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning
    Depositor
    Insert
    human codon optimized Cas9
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pMuLE ENTR SV40-hCas9 L5-L2

    Plasmid
    #62134
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Insert
    human codon optimized Cas9
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  8. pMZ381

    Plasmid
    #59899
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pLSLT

    Plasmid
    #51498
    Purpose
    pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Inserts
    T30 TALEN pair
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
  10. pLSLZ

    Plasmid
    #51495
    Purpose
    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid
    Depositor
    Inserts
    Zif268:FokI
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    SV40 NLS
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. pWPT-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49232
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
  12. AmCyan-P2A-Xerry

    Plasmid
    #48686
    Purpose
    Expresses AmCyan while the mCherry gene has been mutated
    Depositor
    Insert
    AmCyan
    Tags
    mutant mCherry (truncated)
    Expression
    Mammalian
    Mutation
    SV40 NLS
    Promoter
    CMV
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 11201 - 11240 of 18759 results