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pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

Plasmid

#194693

Purpose

CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPD0054_pIVTRup_mCherry2

Plasmid

#242545

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

mCherry

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA Ef1alpha Puro-T2A-GFP

Plasmid

#111596

Purpose

sgRNA Ef1alpha Puro-T2A-GFP

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pVSV eGFP dG

Plasmid

#31842

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-SB-NLS

Plasmid

#228870

Purpose

PE7 connected to MLH1-SB-NLS via 2A sequence

Depositor

Sangsu Bae

Article

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PE7-2A-MLH1-SB-NLS

Use

CRISPR

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin

Plasmid

#227367

Purpose

For bacterial expression of parkin

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression.

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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MP9956:SFG.GD3synthase-2A-GD2synthase

Plasmid

#75013

Purpose

Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

Depositor

Martin Pule

Article

Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

Inserts

murine GD3 synthase
murine GD2 synthase

Use

Retroviral; Momlv retroviral vector

Expression

Mammalian

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Q23

Plasmid

#40261

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP VARP

Plasmid

#42312

Depositor

Thierry Galli

Article

Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

Insert

VARP (ANKRD27 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Tags

GST

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1

Plasmid

#204558

Purpose

Expresses GST-tagged UFBP1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

none

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off blast POLD1

Plasmid

#241438

Purpose

Expresses DOX-suppressible POLD1

Depositor

Richard Possemato

Insert

POLD1 (POLD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Partly codon optimized

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hpGRISZ-TM

Plasmid

#248028

Purpose

Plasmid for packing AAV for hpGRISZ (synaptic zinc sensor)

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2026 Mar 3. doi: 10.1021/acsse

Insert

hpGRISZ

Use

AAV

Promoter

hSyn

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAM233

Plasmid

#226655

Purpose

pcDNA3.1-p97-FLAG

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUG-IRES-mCherry

Plasmid

#222109

Purpose

Start codon reporter (WT AUG)

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

Plasmid

#188383

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-Sec23A

Plasmid

#166892

Purpose

mammalian expression of Sec23A tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-TRE-AFOS

Plasmid

#197079

Purpose

A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

Depositor

Elaine Fuchs

Article

Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

Insert

AFOS (Fos Mouse)

Use

Lentiviral

Tags

Flag and T7

Promoter

TRE

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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