We narrowed to 13,867 results for: cas9

1. pLenti-CRISPRv2-sgNTC49-puro

   Plasmid

   #231989

   Purpose

   Knockout non-targeting control

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 and hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.U6gT28.4.hSyn.H2B.RFP

   Plasmid

   #170387

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH0001

   Plasmid

   #85969

   Purpose

   UCOE-SFFV-dCas9-BFP-KRAB

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BFP-KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   SFFV

   Available Since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2Cre

   Plasmid

   #82415

   Purpose

   Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

   Depositor

   David Feldser

   Article

   Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

   Insert

   Cre Recombinase
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Mutation

   Mutated BsmBI cut site

   Promoter

   EFS (P2A)

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV_Npu-saBE3.9max N-terminal

   Plasmid

   #137179

   Purpose

   Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-saBE3.9max_N-terminal
   

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGES401

   Plasmid

   #190198

   Purpose

   For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PBE-NG

   Plasmid

   #229045

   Purpose

   Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

   Depositor

   Huawei Zhang

   Article

   Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Puro-sgNUAK1

    Plasmid

    #138692

    Purpose

    Expresses a human NUAK1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNUAK1 human (NUAK1 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-CreERT2 sgRipk4 v3

    Plasmid

    #158048

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLKO-CreERT2 sgAdam10 v3

    Plasmid

    #158047

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Adam10 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid

    #106435

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInt-ERCreER

    Plasmid

    #190040

    Purpose

    This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

    Depositor

    Kayoko Tanaka

    Article

    Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

    Insert

    ERT2-Cre-ERT2 flanked by AAVS1
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Puro-sgNUAK2

    Plasmid

    #138693

    Purpose

    Expresses a human NUAK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNUAK2 human (NUAK2 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET42b-HF-BE3

    Plasmid

    #87438

    Purpose

    Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. SunTagng14aa

    Plasmid

    #106439

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LCV2 IRF3 KO

    Plasmid

    #217446

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting IRF3 (IRF3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNICKclos2.0

    Plasmid

    #73228

    Purpose

    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sgRNA to xylR
    

    Use

    E.coli - clostridium shuttle vector

    Mutation

    D10A

    Promoter

    Pj23119 and Pthl

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pX330 Human 3' HP1a gRNA

    Plasmid

    #127907

    Purpose

    WT Cas9 Vector targeting the 3' end of the human HP1a gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    gRNA for Human 3' HP1a
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid

    #202824

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pKDsgRNA-FRT

    Plasmid

    #113398

    Purpose

    expresses gRNA for Cas9 FRT targetting

    Depositor

    Jan Michiels

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    exo, beta, gam, sgRNA-FRT
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. LENTICRISPR-UPP1_sgRNA2

    Plasmid

    #201635

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. plenti-px330-CRBN-T1-pGK-Pur

    Plasmid

    #107382

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRDB_181

    Plasmid

    #216102

    Purpose

    CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiCRISPRv2.sgNf1.1

    Plasmid

    #91895

    Purpose

    sgNf1.1 for Nf1 deletion

    Depositor

    Wen Xue

    Article

    Song et al Gastroenterology. 2017 Apr;152(5):1161-1

    Insert

    sgNf1.1
    

    Use

    Lentiviral and Mouse Targeting

    Expression

    Mammalian

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lentiCRISPRv2.sgNf1.4

    Plasmid

    #92029

    Purpose

    sgNf1.4 for Nf1 deletion

    Depositor

    Wen Xue

    Article

    Song et al Gastroenterology. 2017 Apr;152(5):1161-1

    Insert

    sgNf1.4 for Nf1 deletion
    

    Use

    Lentiviral and Mouse Targeting

    Expression

    Mammalian

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLentiCRISPRv1_SDHB

    Plasmid

    #177981

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHB (SDHB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PXPR007 sgCIC-2

    Plasmid

    #74959

    Purpose

    Cas9 + sgCIC-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-Blast-sgNT

    Plasmid

    #138678

    Purpose

    Expresses a Non-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNT
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRDA_662

    Plasmid

    #216101

    Purpose

    CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSpG-PPE

    Plasmid

    #170130

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Insert

    nCas9-SpG(H840A)-M-MLV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…

    Promoter

    maize Ubiquitin-1

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. A3G-BE5.14

    Plasmid

    #163638

    Purpose

    C to T DNA base editing

    Depositor

    Xue Gao

    Article

    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

    Insert

    A3G-BE5.14
    

    Tags

    BPSV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. A3G-BE5.13

    Plasmid

    #163637

    Purpose

    C to T DNA base editing

    Depositor

    Xue Gao

    Article

    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

    Insert

    A3G-BE5.13
    

    Tags

    BPSV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-Puro-sgSNRK

    Plasmid

    #138694

    Purpose

    Expresses a human SNRK-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSNRK human (SNRK Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLentiCRISPRv1_SDHA

    Plasmid

    #177980

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHA (SDHA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. A3G-BE4.4

    Plasmid

    #163636

    Purpose

    C to T DNA base editing

    Depositor

    Xue Gao

    Article

    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

    Insert

    A3G-BE4.4
    

    Tags

    BPSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge