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We narrowed to 63,680 results for: cloning

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Showing: 5621 - 5640 of 63680 results

  1. pET24a-ncOGT-FL

    Plasmid
    #190821
    Purpose
    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E.coli
    Promoter
    T7
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190821-plasmid-map-sequence-id-379184

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  4. FUGW-APPwt-T2A-mCherry

    Plasmid
    #190803
    Purpose
    Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190803-plasmid-map-sequence-id-374464

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  8. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  14. pMBP-hLIF

    Plasmid
    #179711
    Purpose
    Expresses H6-MBP-LIF
    Depositor
    Mark Arbing
    Insert
    Leukemia inhibitory factor (LIF Human)
    Tags
    His6-MBP-TEV protease site
    Expression
    Bacterial
    Promoter
    tac
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179711-plasmid-map-sequence-id-352198

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  16. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

    Plasmid
    #175523
    Purpose
    Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1-HaloTag11 and Tomm20-HaloTag9
    Expression
    Mammalian
    Mutation
    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    175523-plasmid-map-sequence-id-343758

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  17. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Publication
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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Showing: 5621 - 5640 of 63680 results