We narrowed to 9,109 results for: control

1. pGL3-mFgf21pro2-luc

   Plasmid

   #101798

   Purpose

   Reporter for mouse Fgf21 promoter activity

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   Fibroblast growth factor 21 promoter (Fgf21 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-AICD-IRES-hrGFP

   Plasmid

   #107548

   Purpose

   high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Insert

   AICD (APP Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   human synapsin

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL2 HNF4A P2 wt

   Plasmid

   #60324

   Purpose

   HNF4A P2 promoter cloned into the pGL2-basic backbone.

   Depositor

   Jorge Ferrer

   Article

   Hansen et al J Clin Invest. 2002 Sep;110(6):827-33.

   Insert

   NM_000457.4 (HNF4A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

   Plasmid

   #210710

   Purpose

   This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   M11

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-mFgf21pro3-luc

   Plasmid

   #101799

   Purpose

   Reporter for mouse Fgf21 promoter activity

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   Fibroblast growth factor 21 promoter (Fgf21 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

   Plasmid

   #181850

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SSH-pSUMO-α-COPI-WD40

   Plasmid

   #208341

   Purpose

   Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
   

   Use

   Tags

   His-tag, SUMO-tag, and Strep-tag

   Expression

   Bacterial

   Mutation

   Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-hs-KRAS

   Plasmid

   #52729

   Purpose

   retroviral expression of human KRAS

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-hPKL WT

   Plasmid

   #107159

   Purpose

   For mammalian expression of Flag-hPKL WT

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   Flag-hPKL WT (PKLR Human)
   

   Use

   Retroviral

   Tags

   N-term Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid

    #122985

    Purpose

    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    

    Use

    AAV

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    SspB contains A58V,R73Q "milli" mutatio…

    Promoter

    human synapsin

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. DHC1-AID-Clover donor (Hygro)

    Plasmid

    #158622

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218914

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4330T>C

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8-Magneto2.0-p2A-mCherry

    Plasmid

    #74334

    Purpose

    For gateway cloning Magneto2.0-p2A-mCherry into destination vector

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTH1, FTL, Trpv4 Rat, Human)
    

    Use

    Gateway vector

    Tags

    Flag and p2A-mCherry

    Expression

    Mutation

    TRPV4: delta 760–871

    Promoter

    none

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218910

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

    Plasmid

    #164777

    Purpose

    cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

    Insert

    nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E61C

    Promoter

    pGK

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. DHC1-AID-Clover donor (Neo)

    Plasmid

    #158621

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLH_Scr16

    Plasmid

    #100538

    Purpose

    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Cdc1 NLS, CreC, CreN, and SV40 NLS

    Expression

    Yeast

    Mutation

    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A

    Promoter

    FBA1 and TDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-PHFF-sh-mBVRA

    Plasmid

    #100286

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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