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pCMV-FLAG-LOV*-MVP

Plasmid

#202206

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Major vault protein (MVP Human)

Tags

FLAG and LOV*

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-gfaABC1D-monSTIM1

Plasmid

#161797

Purpose

Lentiviral vector expressing monSTIM1 in astrocytes.

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)

Use

Lentiviral

Tags

EGFP

Promoter

gfaABC1D

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_EGFP-P2A-HAUS6_IRES_Blast

Plasmid

#182886

Purpose

Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)

Depositor

Daniel Gerlich

Article

David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

Insert

HAUS 6 (HAUS6 Human)

Use

Lentiviral

Tags

EGFP

Expression

Bacterial and Mammalian

Promoter

EF1alpha core

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP1-Halo

Plasmid

#175300

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP1 (IGF2BP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Plasmid

#159799

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p5xNF-kb_RE

Plasmid

#118050

Purpose

GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

5 copies of the NF-kb DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-D1R

Plasmid

#106067

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D1R; Opto-D1R; F9; 720)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-D1R

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2

Plasmid

#85119

Purpose

yeast expression of EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Myc-YKT6

Plasmid

#116945

Purpose

Expressing Myc-YKT6 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

Synaptobrevin homolog YKT6 (YKT6 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pROS12

Plasmid

#107926

Purpose

hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-hChibby1

Plasmid

#89770

Purpose

Labels mother centrioles/basal bodies

Depositor

Ken-Ichi Takemaru

Insert

Chibby1 (CBY1 Human)

Use

Lentiviral

Tags

EGFP

Promoter

EF1alpha

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Expression

Mammalian

Mutation

S472A

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
CALNL-DCX-eGFP

Plasmid

#28310

Depositor

Joseph Loturco

Article

Manent et al Nat Med. 2009 Jan . 15(1):84-90.

Insert

Doublecortin (Dcx Rat)

Use

Cre/Lox

Tags

eGFP

Expression

Mammalian

Available Since

April 4, 2011

Availability

Academic Institutions and Nonprofits only
human p53-(1-393)-M340Q-L344R

Plasmid

#24863

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Methionine 340 mutated to Glutamine Leucine 344 …

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pTYF-2xGfaABC1D-tTA

Plasmid

#19981

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xGfaABC1D-tTA

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only

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pTYF-1xSYN-EGFP

Plasmid

#19973

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

SYN-EGFP-WPRE (SYN1 Human, jellyfish)

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (4x TBE1 - mut)

Plasmid

#16598

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE1 mut) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant TBE1 sites

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only