We narrowed to 9,384 results for: Coli

1. pSG28-J23151-AraC

   Plasmid

   #102453

   Purpose

   Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   AraC (araC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23151

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MK1283

   Plasmid

   #71428

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Use

   Tags

   Expression

   Mutation

   T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

   Promoter

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-c5X-ELP[V-60]

   Plasmid

   #67013

   Purpose

   Expresses malE-ELP[V-60] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-ELP[V-60]
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB1C3-J23110-B0034-mRFP1_Violet

   Plasmid

   #160447

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   promoter, RBS, mRFP1E_Violet
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKFSS1_2

   Plasmid

   #118227

   Purpose

   Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PtxS1-c003

   Plasmid

   #173076

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PtxS1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D35-I221

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pet28a-His-tag-BS2

   Plasmid

   #220347

   Purpose

   E. coli expression vector to purify BS2

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBSV2G_2

   Plasmid

   #118225

   Purpose

   Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST17-UnaG

   Plasmid

   #203485

   Purpose

   For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Salmonella MTAN

    Plasmid

    #64041

    Purpose

    Expression of S. enterica MTAN in E. coli

    Depositor

    Vern Schramm

    Article

    Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

    Insert

    Methylthioadenosine nucleosidase
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pOGG005

    Plasmid

    #113980

    Purpose

    Level 1 high copy number vector for E. coli expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB1C3-J23110-B0034-mRFP1E

    Plasmid

    #160442

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSETb-Ferritin-Dronpa

    Plasmid

    #101194

    Purpose

    Expresses a Ferritin-Dronpa fusion construct in E. coli

    Depositor

    Peter Dedecker

    Article

    Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

    Insert

    Ferritin-Dronpa
    

    Use

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-T20S

    Plasmid

    #110805

    Purpose

    expresses Thermoplasma acidophilium 20S proteasome in E. Coli

    Depositor

    Yifan Cheng

    Article

    Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

    Inserts

    PsmB
    PsmA
    

    Use

    Tags

    tev-His

    Expression

    Bacterial

    Mutation

    N2D and Q2E

    Promoter

    T7 and T7 promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b_SNAP-ecDHFR

    Plasmid

    #187108

    Purpose

    Expression of SNAP-ecDHFR fusion in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    SNAP-ecDHFR
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUMV19

    Plasmid

    #67161

    Purpose

    inducible operation of the mevalonate pathway in E. coli

    Depositor

    Tomohisa Kuzuyama

    Article

    Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

    Insert

    the mevalonate pathway genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a_LolT_C41S

    Plasmid

    #211789

    Purpose

    Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

    Depositor

    Yang Hai

    Article

    Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

    Insert

    lolt mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-6xHis-GFP(+6)

    Plasmid

    #199160

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+6)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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