We narrowed to 51,141 results for: ANG

1. pDEST-APLO

   Plasmid

   #112805

   Purpose

   gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

   Depositor

   Chun Han

   Article

   Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. hfDECOR-F148A

   Plasmid

   #219546

   Purpose

   Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-F148A
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed TadA8e Phenylalanine 148 to Alanine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hfDECOR-V106G

   Plasmid

   #219547

   Purpose

   Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-V106G
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed TadA8e Valine 106 to Glycine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ230-D156R (ttLbCas12a)

   Plasmid

   #195366

   Purpose

   A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   LbCas12a-D156R (ttLbCas12a)
   

   Use

   CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

   Expression

   Plant

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. px458_Conc2

   Plasmid

   #134449

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUS_B4

   Plasmid

   #31021

   Depositor

   Daniel Voytas, Adam Bogdanove

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSET-dCas9-actII-4-NT-S1

   Plasmid

   #110185

   Purpose

   Repression of gene expression in Streptomyces by CRISPRi

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Inserts

   dCas9
   sgRNA
   

   Use

   CRISPR; I

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   J23119 and ermE*p

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETNb-nALFA

   Plasmid

   #159988

   Purpose

   For the protein expression with ALFA tag at N-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

   Expression

   Bacterial

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW170b2

   Plasmid

   #136929

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gVI
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sc AAV miR26a eGFP

    Plasmid

    #21894

    Depositor

    Joshua Mendell

    Article

    Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

    Insert

    EF1 alpha -miR26a GFP p(A)
    

    Use

    AAV; Sc

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUS_B6

    Plasmid

    #31023

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUS_B5

    Plasmid

    #31022

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. px458_Conc3

    Plasmid

    #134450

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUS_B3

    Plasmid

    #31020

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUS_B1

    Plasmid

    #31018

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. px459V2.0_Conc3

    Plasmid

    #134458

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHA-PUMA DeltaBH3

    Plasmid

    #16589

    Depositor

    Bert Vogelstein

    Article

    Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

    Insert

    PUMA (BBC3 Human)
    

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    BH3 domain deleted

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTKhc-yibDp-GFP-cp6

    Plasmid

    #134938

    Purpose

    Low phosphate inducible circular permutation of GFP

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPcp6
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP_sfCherry2(11)_Keratin

    Plasmid

    #82604

    Purpose

    Expresses sfCherry2(11) tagged keratin in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_Keratin
    

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. LMAPCW

    Plasmid

    #205655

    Purpose

    shRNAmir backbone for RNA interference, with mCherry reporter

    Depositor

    Dapeng Wang

    Article

    Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

    Insert

    miR-AB
    

    Use

    Lentiviral

    Promoter

    mCMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SAFB1-pcDNA3.1

    Plasmid

    #32742

    Depositor

    Steffi Oesterreich

    Article

    Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

    Insert

    SAFB1 (SAFB Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV5B-Flag-Par6 wt

    Plasmid

    #11748

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    PAR6 (Pard6a Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

26. LMAPPW

    Plasmid

    #205657

    Purpose

    shRNAmir backbone for RNA interference, with Puromycin selection

    Depositor

    Dapeng Wang

    Article

    Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

    Insert

    miR-AB
    

    Use

    Lentiviral

    Promoter

    mCMV

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV TBG FFLuc

    Plasmid

    #35658

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TBG

    Available Since

    July 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSUMO His6 SUMO AMPKa (11-281)

    Plasmid

    #177851

    Purpose

    Bacterial Expression of AMPKa1

    Depositor

    Karsten Melcher

    Article

    Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

    Insert

    AMPK a1 (PRKAA1 Human)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBI-EGFP-PUMA

    Plasmid

    #16590

    Depositor

    Bert Vogelstein

    Article

    Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

    Insert

    PUMA (BBC3 Human)
    

    Tags

    2x HA

    Expression

    Mammalian

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pLenti‐puro‐OgRNA_mdxE53

    Plasmid

    #187054

    Purpose

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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