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GP734

Plasmid

#164527

Purpose

Expresses Flag-Ub

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin

Use

Lentiviral

Tags

FLAG and Ub

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Publication

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBTK599

Plasmid

#110600

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAtU6-sgRNA

Plasmid

#119775

Purpose

Expression of a target sgRNA in potato

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

AtU6-SmR-sgRNA

Expression

Plant

Promoter

AtU6

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYZ033

Plasmid

#98404

Purpose

Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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psgRNA2.02-PP7-ccdB

Plasmid

#99912

Purpose

Basic cloning vector for the expression of PP7 inserted sgRNA

Depositor

Yujie Sun

Publication

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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CMV_3xFLAG_NLS_ISCro4_NLS_T2A_puroR_SV40_polyA

Plasmid

#251494

Purpose

Expression of a human codon optimised ISCro4 bridge recombinase

Depositor

Martin Jinek

Publication

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge recombinase

Expression

Mammalian

Mutation

NA

Available since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CK8e_Cas9_miniPA

Plasmid

#226141

Purpose

AAV construct for HITI insert with CK8e promoter and SpCas9

Depositor

Christopher Nelson

Publication

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Cas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CK8e

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE46

Plasmid

#90384

Purpose

NFYA - constitutive enhancer gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NF-Y

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE130

Plasmid

#118484

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBLADE_ONLY_A

Plasmid

#168051

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

Depositor

Barbara Di Ventura

Publication

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Insert

Blue light-inducible AraC dimers in Escherichia coli

Expression

Bacterial

Promoter

J23101**

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKAd5f11p-EPG

Plasmid

#122697

Purpose

To generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.

Depositor

Zhuo-Zhuang Lu

Publication

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Insert

GFP

Use

Adenoviral

Promoter

EF1a

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE155

Plasmid

#118505

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-PafA-XIAP

Plasmid

#113402

Purpose

Expresses PafA-XIAP in bacteria

Depositor

Min Zhuang

Publication

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

PafA-XIAP

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A0108

Plasmid

#91001

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AtCas9

Use

CRISPR

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBS35

Plasmid

#83797

Purpose

Useful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selection

Depositor

Eric Muller

Publication

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

mCherry

Tags

mCherry

Expression

Yeast

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAL-pgi_NT

Plasmid

#74067

Purpose

pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR

Depositor

Timothy Lu

Publication

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pgi (NT)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBadcrtEBI_13

Plasmid

#63811

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Publication

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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bfloGFPc1a

Plasmid

#60750

Purpose

Bacterial expression of Amphioxus GFPc1

Depositor

Dimitri Deheyn

Publication

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPc1

Tags

8xHIS

Expression

Bacterial

Promoter

T7

Available since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
pαH-S-GSAS-OMICRON

Plasmid

#180423

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

Depositor

Priyamvada Acharya

Publication

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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