We narrowed to 14,011 results for: flag

1. pLV_ADRB2-mTurquoise2-iLID

   Plasmid

   #161002

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   ADRB2-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. NES-Halo

   Plasmid

   #200201

   Purpose

   Expression of 3X HIV NES

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   3X HIV Rev NES-Halo
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF_1alpha

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK7-Rheb

   Plasmid

   #15888

   Depositor

   John Blenis

   Article

   Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

   Insert

   Rheb (RHEB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pDest490-OPN-a

   Plasmid

   #17590

   Depositor

   Xin Wang

   Article

   Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

   Insert

   OPN-a (SPP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Base pairs 49–942

   Promoter

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pME18S-F-R-Olfr160

   Plasmid

   #149366

   Purpose

   Expresses Olfr160 in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

   Insert

   Olfr160 (Olfr160 Mouse)
   

   Use

   Tags

   FLAG and rhodopsin

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EdTC

   Plasmid

   #24310

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   31 first amino acids of hTcf4 have been deleted.

   Promoter

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScI_dCas9-CDA

   Plasmid

   #108549

   Purpose

   Bacterial Target-AID vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcpH-TM

   Plasmid

   #85389

   Purpose

   Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses

   Depositor

   Gregory Melikyan

   Article

   Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

   Insert

   ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST1374

   Plasmid

   #13426

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK2014

    Plasmid

    #171931

    Purpose

    To express sygl-1 fused to 24 copies of MS2 loops.

    Depositor

    Judith Kimble

    Article

    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

    Insert

    Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
    

    Use

    Tags

    N-terminal Tag 24X MS2 loops

    Expression

    Worm

    Mutation

    Promoter

    -

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-RILP

    Plasmid

    #74600

    Purpose

    used for expression in E. coli and purification

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab-interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB1H1

    Plasmid

    #12611

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Dorsal RHR in pB1H1 (dl Fly)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB701NL3F10H

    Plasmid

    #141288

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFETCh_ZBTB1

    Plasmid

    #128235

    Purpose

    Donor vector for 3' FLAG tag of human ZBTB1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiN 16E6no*

    Plasmid

    #37445

    Purpose

    Lentiviral expression of HPV 16E6no* in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 16E6no* (E6 HPV)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    V42L

    Promoter

    CMV

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pDest490-OPN-c

    Plasmid

    #17592

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-c (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 1–93, 175–942

    Promoter

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJM60-Cnbp

    Plasmid

    #38246

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Cnbp (Cnbp Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSplit-CO-hDib1

    Plasmid

    #58788

    Purpose

    Express LucC-hDib1 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pZZMP122

    Plasmid

    #231756

    Purpose

    Mammalian expression of SPO11-YF

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    SPO11-YF (Spo11 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y138F

    Promoter

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pZZMP154

    Plasmid

    #231760

    Purpose

    Mammalian expression of LZ-SPO11

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    LZ-SPO11 (Spo11 Mouse, Budding Yeast)
    

    Use

    Tags

    Flag-LeucineZipper

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pVITRO1-SS-338

    Plasmid

    #68795

    Purpose

    Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-Terminal Sensor 1 V1
    mCherry
    ZF9 Op x6
    EGFP
    N-Terminal Sensor 1 V2
    

    Use

    Tags

    IRES, NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pKAR1/Pur

    Plasmid

    #23105

    Purpose

    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance gene

    Depositor

    Randy Poon

    Article

    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE for cDNA; U6 for shRNA

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

25. lentiMutB3GNT5-blast

    Plasmid

    #208380

    Purpose

    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nt 922 c->g (PAM site inactivation)

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCDNA-FRT-FAK30

    Plasmid

    #59121

    Purpose

    FAK fret sensor with 30 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNCMV 16E6no*

    Plasmid

    #37454

    Purpose

    Expression of HPV 16E6no* in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 16E6no* (E6 HPV)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    V42L

    Promoter

    CMV

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pSPlit-OC-hDib1

    Plasmid

    #58789

    Purpose

    Express hDib1-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pSplit-ON-hDib1

    Plasmid

    #58787

    Purpose

    Express hDib1-LucN fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (1-398aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

30. PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

    Plasmid

    #174526

    Purpose

    Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Insert

    PylS
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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