We narrowed to 10,848 results for: ENA

1. anti-PSD-95 scFv [K28/43] with sortase tag

   Plasmid

   #204422

   Purpose

   Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

   Insert

   Recombinant mouse scFv targeting PSD-95 (Homo sapiens)
   

   Use

   Tags

   6xHis tag and Sortase tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LSB-hsa-miR-133b

   Plasmid

   #103223

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-133b target (MIR133B Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs_MCART1

   Plasmid

   #133253

   Purpose

   Express untagged MCART1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   MCART1 (SLC25A51 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells and…

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. iSH-iRFP670-sspB

   Plasmid

   #176102

   Purpose

   Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   phosphatidylinositol 3-kinase (Pik3r1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

   Plasmid

   #105036

   Purpose

   Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-RFP-IKZF1-sh3

   Plasmid

   #69042

   Purpose

   Lentiviral expression of IKZF1 shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1 (IKZF1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPXL-Klf4

   Plasmid

   #36981

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

8. plenti-px330-CRBN-T2-pGK-Pur

   Plasmid

   #107383

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP scFv [N86/8]

   Plasmid

   #199429

   Purpose

   Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targetingGFP (Aequorea victoria)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1 KanR foundation lox casette

    Plasmid

    #132388

    Purpose

    Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    G418 resistance
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    promoterless

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Met1 diUb (1-152)

    Plasmid

    #110766

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTXB1-EXO1b D173A

    Plasmid

    #68269

    Purpose

    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

    Depositor

    Stefano Ferrari

    Article

    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    catalytically-dead D173A. Please see depositor co…

    Promoter

    T7

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3.1 hSCN5A-L325R

    Plasmid

    #145373

    Purpose

    expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 325 to Arginine

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPR v2-sgAMPK alpha2 clone1

    Plasmid

    #162122

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha2

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MCP-3x9aaTAD

    Plasmid

    #210707

    Purpose

    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBC2T-actin

    Plasmid

    #108968

    Purpose

    expresses BC2-tag-actin in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    beta-actin (ACTB Human)
    

    Use

    Tags

    BC2-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs_FLAG-MCART1K91A

    Plasmid

    #133248

    Purpose

    Express FLAG-tagged MCART1K91A in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells, K9…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid

    #208967

    Purpose

    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSB-hsa-miR-195-3p

    Plasmid

    #103311

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-195-3p target (MIR195 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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