We narrowed to 146,921 results for: ins;

1. GFP-PKI nls

   Plasmid

   #118301

   Purpose

   Expression of PKIa

   Depositor

   Philip Stork

   Article

   Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

   Insert

   PKI nls (aa 2-36)
   

   Use

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   inserting 2xnls

   Promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Stat6-SH2

   Plasmid

   #46517

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat6 (STAT6 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 510-654

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Tec-SH2

   Plasmid

   #46522

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Tec (TEC Human)
   

   Use

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 236-351

   Promoter

   Tac

   Available Since

   Oct. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

   Plasmid

   #72888

   Purpose

   fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

   Insert

   Gaussia luciferase, Channelrhodopsin-2, EYFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KCC2B_HUMAN_D0

   Plasmid

   #79718

   Purpose

   This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KCC2B
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ZIKV_Sensor_32B_LacZ

   Plasmid

   #75007

   Purpose

   Toehold switch sensor to detect Zika Virus

   Depositor

   James Collins, Alexander Green

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_sensor_32B_LacZ
   

   Use

   Tags

   LacZ

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(+) 6xHis-TEV-IFIT3

   Plasmid

   #53559

   Purpose

   bacterial expression of IFIT3

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT3 (IFIT3 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMS1: pHelper(AvCAST)_AvPSP1

   Plasmid

   #168133

   Purpose

   Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   AvTnsD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-CreLite

    Plasmid

    #131782

    Purpose

    Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    Cre/Lox; Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CaMKIIa-Mac 3.0-EYFP

    Plasmid

    #35515

    Purpose

    Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eMac 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking signal, ER export

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCellulose

    Plasmid

    #36300

    Depositor

    Jay Keasling

    Article

    Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

    Insert

    pCellulose
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Two promoters from the E coli genome drive two di…

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP2253

    Plasmid

    #70704

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5 MEKK4alpha KD

    Plasmid

    #12188

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Kinase dead: K1361M

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

15. Cry2PHR.mCh.CofS3E

    Plasmid

    #133619

    Purpose

    Expresses Cry2PHR - mCherry - CofilinS3E protein fusion

    Depositor

    Robert Hughes

    Article

    Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

    Insert

    Cry2PHR - mCherry - CofilinS3E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MK14_HUMAN_D0

    Plasmid

    #79680

    Purpose

    This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK14
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pEAK-cKal7

    Plasmid

    #145801

    Purpose

    expresses Kalirin-7 with the c-front in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

    Insert

    cKalirin-7 (Kalrn Rat)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    EIF2a

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. ZIKV_Trigger_27B

    Plasmid

    #75008

    Purpose

    Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_27B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS18: pHelper(PmcCAST)_PmcPSP1

    Plasmid

    #168151

    Purpose

    Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    PmcTniQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSP3-bio

    Plasmid

    #47731

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP3
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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