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pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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(364) SB KRAB-SadCas9

Plasmid

#163022

Purpose

Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Promoter

TET-On

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP (PV2527)

Plasmid

#99039

Purpose

AAV-mediated expression of ArchT under the CamKII promoter

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLVPT-GDNF-rtTR-KRAB-2SM2

Plasmid

#11647

Purpose

Tet-regulated (Tet-off) lentiviral vector for GDNF (mPGK promoter) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GDNF, rtTR-KRAB-2SM2, Tet-Off (GDNF Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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YFP-mDia2

Plasmid

#25420

Depositor

Arthur Alberts

Article

Wallar et al J Biol Chem. 2006 Feb 17. 281(7):4300-7.

Insert

diap3 (Diaph3 Mouse)

Tags

YFP A206K

Expression

Mammalian

Mutation

Encodes mDia2 amino acids T47 to L1171

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLVTHM-shRnd3

Plasmid

#86437

Purpose

2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

Depositor

Violaine Moreau

Article

Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

Insert

shRNA targeting RND3 (RND3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP hBub1 K821R

Plasmid

#59813

Purpose

Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

K821R

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep164

Plasmid

#136873

Purpose

Mammalian expression of the centrosomal protein Cep164 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep164 (CEP164 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-E12

Plasmid

#25990

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pAL130

Plasmid

#22475

Purpose

Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF3NT (PIF3 Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pICSL11055

Plasmid

#68252

Purpose

Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos

Use

Synthetic Biology

Expression

Plant

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-MyoD

Plasmid

#25992

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-MyoD homodimer (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO GFP hBub1 WT

Plasmid

#59812

Purpose

Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Tags

EmGFP

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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p3s-ABE7.10

Plasmid

#113128

Purpose

expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE 7.10

Tags

HA epitope and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pF153 pcDNA3.1(+)SOD1G37R

Plasmid

#26399

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G37R

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pblic-neo TRX1

Plasmid

#110919

Purpose

To overexpress Thioredoxin-1

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Retroviral

Promoter

unknown

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSMP-Ehmt1_2

Plasmid

#36336

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only