We narrowed to 63,598 results for: cell

1. pTT3-SP-6xHis-TIE1(FL)-FLAG

   Plasmid

   #157621

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   TIE1 (TIE1 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Donor_YTHDF2_KO_Neo

   Plasmid

   #186672

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Neomycin (YTHDF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Donor_ANKRD1_KO_Hygro

   Plasmid

   #186668

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Hygromycin (ANKRD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 330-BFP-hCYP3A4-enhancer-R-sgRNA

   Plasmid

   #176841

   Purpose

   Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer right boundary
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cry2 mcherry Gamma 9 in pcDNA3.1

   Plasmid

   #64208

   Purpose

   mCherry fused between Cry2 and gamma 9 to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 (CRY2 Mustard Weed)
   gamma cone (GNGT2 Human)
   

   Use

   Tags

   mcherry and n/a

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pX459_MCM2_sgRNA

   Plasmid

   #232730

   Purpose

   Generates MCM2 dTAG C ternimal knock-in cell lines

   Depositor

   Junjie Chen

   Insert

   MCM2 (MCM2 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NO

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459_CDC45_sgRNA

   Plasmid

   #232732

   Purpose

   Generates CDC45 dTAG C ternimal knock-in cell lines

   Depositor

   Junjie Chen

   Insert

   CDC45 (CDC45 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NO

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-hCCDC11

   Plasmid

   #122993

   Purpose

   Expresses Flag-tagged human CCDC11 in mammalian cells

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Insert

   human CCDC11 (CFAP53 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-MTS-TFAM-V5

   Plasmid

   #200813

   Purpose

   expresses MTS-TFAM in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   Transcription Factor A, Mitochondrial (TFAM Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-FLAG-BubR1

    Plasmid

    #200814

    Purpose

    expresses BubR1 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX ALIX-GFP S718/721/E

    Plasmid

    #138584

    Purpose

    Expresses fluorescent ALIX S718/721E in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    changed serines 718 and 721 to glutamic acids

    Promoter

    TREtight

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest_U6_A2_CG2_T1snp_gRNA

    Plasmid

    #213789

    Purpose

    pDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNA

    Depositor

    William Talbot

    Insert

    nrg1 type I SNP sgRNA (nrg1 Zebrafish)
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157024

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    JAG1 (JAG1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCDH-WT-ALKBH5-3xFLAG

    Plasmid

    #203736

    Purpose

    Overexpression of ALKBH5-WT

    Depositor

    Jianjun Chen

    Article

    Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

    Insert

    ALKBH5 (ALKBH5 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAd-M3cherry

    Plasmid

    #61041

    Purpose

    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.

    Depositor

    Qiao Zhou

    Article

    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.

    Inserts

    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156912

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    IGSF8 (IGSF8 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PCDH-Mut-ALKBH5-3xFLAG

    Plasmid

    #203737

    Purpose

    Overexpression of ALKBH5-Mut

    Depositor

    Jianjun Chen

    Article

    Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

    Insert

    ALKBH5 (ALKBH5 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Enzyme Inactive Mutation (H226A)

    Promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156543

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PDCD1 (PDCD1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD649-HAsp-MERTK-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156878

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    MERTK (MERTK Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD649-HAsp-CD276-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156861

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CD276 (CD276 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge