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pSR11

Plasmid

#69154

Purpose

read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
fzr-1 (fzr-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

fzr-1 and rps-27

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD4_3

Plasmid

#36376

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD4 (MBD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD4_2

Plasmid

#36375

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD4 (MBD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00305

Plasmid

#34431

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10021 (bowl Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pTBK1

Plasmid

#87443

Purpose

express TBK1 in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

TBK1 (Tbk1 Mouse)

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN-PAmCherry-KFERQ-NE

Plasmid

#102365

Purpose

Lentiviral overexpression of PA-mCherry-KFERQ fusion

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Inserts

PA-mCherry
KFERQ peptide

Use

Lentiviral

Tags

NE

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Tau(WT)

Plasmid

#187023

Purpose

Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pARC8-LambdaRedBeta-EcSSB

Plasmid

#162572

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB

Expression

Bacterial

Promoter

pBAD

Available Since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-EF1α-mKate2-biPAC

Plasmid

#169127

Purpose

AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

Depositor

Georg Nagel

Article

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Insert

mKate2-biPAC

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

codon optimized to mouse

Promoter

EF1a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTXB1-pA-M.EcoGII

Plasmid

#192873

Purpose

pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

Depositor

Chong Tang

Article

Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

Insert

pA-M.EcoGII

Tags

3X Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHDR_NANOS3-T2A-mVenus-PuroTK

Plasmid

#222903

Purpose

Homology directed repair template for knocking in mVenus reporter to NANOS3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mVenus

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pREC1010lac-Kva1

Plasmid

#240695

Purpose

RSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Kva1 RT,Kva1 ncRNA, Beta and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010lac-Eas1

Plasmid

#240696

Purpose

RSF1010 origin of replication plasmid containing Eas1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eas1 RT, Eas1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010lac-Ban1

Plasmid

#240693

Purpose

RSF1010 origin of replication plasmid containing Ban1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Ban1 RT, Ban1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010lac-Ebu1

Plasmid

#240699

Purpose

RSF1010 origin of replication plasmid containing Ebu1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-FlaviA-GFP-puro

Plasmid

#140088

Purpose

Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

FlaviA-GFP

Use

Lentiviral; Low expression

Tags

6XHis tag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-SOX4

Plasmid

#51304

Purpose

Expression of SOX4 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

SRY (sex determining region Y)-box 4 (SOX4 Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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