We narrowed to 9,384 results for: Coli

1. pE5772

   Plasmid

   #170779

   Purpose

   expression of hybrid proteins in E. coli

   Depositor

   Anthony Schryvers

   Article

   Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

   Insert

   GentR
   

   Use

   Tags

   polyhisbioMbpTEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSecEffector wo TEVp

   Plasmid

   #172892

   Purpose

   atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   Bxb1
   TP901
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   nTETO and pBAD /AraC

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest-894 (attR4-attR2)

   Plasmid

   #161893

   Purpose

   Multisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coli

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPMY1CB0001

   Plasmid

   #162317

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPQD0KN0025

   Plasmid

   #162283

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSUI-SynP-CAS1sg

   Plasmid

   #154341

   Purpose

   Expression of the sgRNA targeting to the E. coli can gene

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Insert

   CAS1 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSUI-SynP-CAS2sg

   Plasmid

   #154342

   Purpose

   Expression of the sgRNA targeting to the E. coli can gene

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Insert

   CAS2 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUI-SynP-CANTsg

   Plasmid

   #154343

   Purpose

   Expression of the sgRNA targeting to the E. coli can gene

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Insert

   CANT sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSUI-SynP-CAPsg

   Plasmid

   #154344

   Purpose

   Expression of the sgRNA targeting to the E. coli can gene

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Insert

   CAP sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-SznF E215A

    Plasmid

    #139565

    Purpose

    expresses SznF E215A in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E215A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 215 to alanine

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-SznF

    Plasmid

    #139564

    Purpose

    expresses SznF in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAW092

    Plasmid

    #113250

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas1 H205A

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas1
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    H205A

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbA5k-AcrB/AcrD/AcrFa, ABO_0964

    Plasmid

    #86202

    Purpose

    Expresses efflux pump in E. coli to provide resistance to biofuel (medium copy)

    Depositor

    Mary Dunlop

    Article

    Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

    Insert

    Efflux pump
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHL 309

    Plasmid

    #64906

    Purpose

    KanR + ColE1 + PLlacO-1::RBS(st7):rfp::RBS(st7):cfp::RBS(st7):yfp::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pHL 1988

    Plasmid

    #64908

    Purpose

    KanR + ColE1 + PLlacO-1::RBS(st7):cfp::Asp terminator + PLlacO-1::RBS(st7):psu::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCVD453

    Plasmid

    #66154

    Purpose

    cloning of E. coli E2348/69 left end of the LEE region, contains rorf1 and espG.

    Depositor

    James Kaper

    Article

    McDaniel et al Proc Natl Acad Sci U S A. 1995 Feb 28;92

    Insert

    LEE probe A rorf1, espG
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pJT104

    Plasmid

    #25088

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    Edge detector delta luxI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. enz.043-SETD7-Y305F

    Plasmid

    #154045

    Purpose

    Expresses SETD7 (Y305F) in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Histone-lysine N-methyltransferase SETD7 (SETD7 Human)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    changed tyrosine 305 to phenylalanine

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAKW.119-R5-TEV-SUMO-His

    Plasmid

    #154050

    Purpose

    Expresses R5 peptide in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Silaffin R5 peptide
    

    Use

    Tags

    SUMO and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5LacO

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAKW.111A-SETD7

    Plasmid

    #154052

    Purpose

    Expresses SETD7 (Y305F) in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Histone-lysine N-methyltransferase SETD7 (SETD7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed tyrosine 305 to phenylalanine

    Promoter

    pTet*

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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