159,530 results

1. pET22b-CPDBamHI-Leu

   Plasmid

   #38256

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Neo-Bam APC 1-1941

   Plasmid

   #16510

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon 1941 truncation

   Promoter

   Available since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. PSMA4_GFP_Cterm

   Plasmid

   #221514

   Purpose

   Fluorescently tagged Psma4-GFP

   Depositor

   Alessandro Ori

   Insert

   PSMA4 (PSMA4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-hSNCA-GFP

   Plasmid

   #215489

   Purpose

   Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRPN265NB-LaG16-MNase

   Plasmid

   #170978

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

   Insert

   Glutathione-S-Transferase/LaG16/MNase
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. sfGFP-pBAD

   Plasmid

   #54519

   Purpose

   Localization: Protein Expression Vector , Excitation: 485, Emission: 507

   Depositor

   Michael Davidson, Geoffrey Waldo

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   6xHis and SfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

   Plasmid

   #176628

   Purpose

   TF A self-activation cassette in MultiFate-2.3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209666

   Purpose

   Non-responsive control

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

   Plasmid

   #121538

   Purpose

   An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

   Depositor

   Christopher Richie

   Article

   Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

   Has Service

   AAV2 and AAV5

   Insert

   HA-tagged hM4D(Gi) (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SYN1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCP30T-E2Crimson

    Plasmid

    #78478

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    E2-Crimson
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSM-GFPnovo2

    Plasmid

    #116943

    Purpose

    GFPnovo2_unc-54 utr vector for expression in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Hendi et al MicroPubl Biol. 2018 Sep 6;2018:10.17912

    Insert

    C. elegans codon optimized GFPnovo2
    

    Use

    Tags

    GFPnovo2

    Expression

    Worm

    Mutation

    Y145F, V163A, S202T, L221V

    Promoter

    none

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pP450cam

    Plasmid

    #85085

    Purpose

    Expresses P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. NylCK_pET28a

    Plasmid

    #215422

    Purpose

    Expression construct for NylCK gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN1-CMV-mCherryN-IntN-DrBphP (N23)

    Plasmid

    #210724

    Purpose

    NIR optogenetic module for conditional protein splicing of split mCherry (N-part)

    Depositor

    Vladislav Verkhusha

    Article

    Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

    Insert

    N23
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIRT2 Flag

    Plasmid

    #13813

    Purpose

    Mammalian expression of human SIRT2 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Transcript variant 2

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. wtTDP43tdTOMATOHA

    Plasmid

    #28205

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    HA tag and tdtomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-HA-USP43

    Plasmid

    #22583

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP43 (USP43 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    not FL, missing first 108 aa

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD44S pBabe-puro

    Plasmid

    #19127

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 standard (CD44 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid

    #188902

    Purpose

    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-KRAB(Kox1)-MeCP2
    

    Use

    Lentiviral

    Tags

    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLVX-CMV-Galpha-q ONE-GO

    Plasmid

    #189738

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-q ONE-GO
    GNAQ (Gnaq Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLEX_306

    Plasmid

    #41391

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-RAD50

    Plasmid

    #116784

    Purpose

    Lentiviral expression of RAD50

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAD50 (RAD50 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CDH5-AbR-GFP

    Plasmid

    #122970

    Purpose

    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

    Depositor

    Budd A. Tucker

    Inserts

    CDH5p
    CopGFP
    Zeocin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

    Plasmid

    #229770

    Purpose

    Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
    

    Use

    Luciferase

    Tags

    LgBiT

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid

    #229769

    Purpose

    Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    

    Use

    Luciferase

    Tags

    SmBiT

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGWB506

    Plasmid

    #74848

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-DIO-dAPEX2

    Plasmid

    #117174

    Purpose

    Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    dAPEX2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF3334

    Plasmid

    #144810

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP100 (SP100 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF0969

    Plasmid

    #141770

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge