We narrowed to 54,588 results for: plasmid

1. COVID-SARS2 NSP16/10

   Plasmid

   #159615

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

   Depositor

   Opher Gileadi

   Inserts

   Covid-SARS2 Nsp16
   Covid-SARS2 Nsp10
   

   Tags

   His6-Zb-TEV and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJA281

   Plasmid

   #22513

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   Pmex-5::mCherry (w introns w/o stop)
   

   Expression

   Worm

   Available Since

   March 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJA256

   Plasmid

   #21509

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   (Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
   

   Use

   Multisite gateway

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pE_Cre_lambdaCos

   Plasmid

   #173785

   Purpose

   The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   Cre
   

   Use

   Cre/Lox and Synthetic Biology

   Mutation

   S102Y and S279Y- please see depositor comments

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJA304

   Plasmid

   #28091

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
   

   Expression

   Worm

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. SJZ7-KAN

   Plasmid

   #228260

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   kan
   

   Expression

   Yeast

   Promoter

   GPM1 S.c.

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SJZ4-NAT

   Plasmid

   #228257

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   nat1
   

   Expression

   Yeast

   Promoter

   FBA1 S.c.

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT27

   Plasmid

   #204308

   Purpose

   For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-GFPc
   

   Use

   CRISPR

   Promoter

   MHC

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT18

   Plasmid

   #204307

   Purpose

   For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-GFPn
   

   Use

   CRISPR

   Promoter

   MHC

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4133227

    Plasmid

    #152132

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pJA245

    Plasmid

    #21506

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    pmex-5::GFP (w introns)::(Gly)5Ala
    

    Use

    Multisite gateway

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4137386

    Plasmid

    #149540

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
    

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

13. MCV

    Plasmid

    #81174

    Purpose

    MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJA269

    Plasmid

    #21544

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    phsp16.41::GFP-F64LS65T::(Gly)5Ala
    

    Use

    Multisite gateway

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBACgus N-His/GST/LRRK2 1867-2186

    Plasmid

    #40403

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGBW-m4046203

    Plasmid

    #145488

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGBW-m4134900

    Plasmid

    #151911

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    d1-15 d1209-1273 R682A R685G K986P V987P

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGBW-m4046233

    Plasmid

    #149032

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4252984

    Plasmid

    #153898

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCJ155

    Plasmid

    #175054

    Purpose

    psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

    Depositor

    David Nielsen

    Article

    Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

    Insert

    CRE
    

    Use

    Unspecified

    Promoter

    rbcLXS

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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