We narrowed to 4,504 results for: pcr

1. pFA6a-6xGLY-Strep-tagII-HIS3MX6

   Plasmid

   #20756

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-6xGLY-His-tag-HIS3MX6

   Plasmid

   #20762

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-6xGLY-Strep-tagII-hphMX4

   Plasmid

   #20758

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-6xGLY-Strep-tagII-kanMX6

   Plasmid

   #20757

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-6xGLY-His-tag-hphMX4

   Plasmid

   #20764

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-6xGLY-S-tag-kanMX6

   Plasmid

   #20766

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-6xGLY-VSV-G-hphMX4

   Plasmid

   #20773

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-6xGLY-S-tag-hphMX4

   Plasmid

   #20767

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-6xGLY-S-tag-HIS3MX6

   Plasmid

   #20765

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-6xGLY-VSV-G-kanMX6

    Plasmid

    #20772

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pES133

    Plasmid

    #127552

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Insert

    F5-flanked tetracycline resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Multiple SNPs in tetA (originally from pBR322) to…

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-HBH-kanMX6

    Plasmid

    #26872

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HBH tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-HTB-kanMX6

    Plasmid

    #26875

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HTB tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-HBH-hphMX4

    Plasmid

    #26873

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HBH-tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-HTB-TRP1

    Plasmid

    #26877

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HTB tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-HBH-TRP1

    Plasmid

    #26874

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HBH tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-RGS18HIS-hphMX4

    Plasmid

    #26883

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    18xHis tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-HTB-hphMX4

    Plasmid

    #26876

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HTB tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-RGS18HIS-kanMX6

    Plasmid

    #26882

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    18xHis tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-BIO-hphMX4

    Plasmid

    #26880

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    BIO-tag
    

    Use

    Pcr-tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFA6a-BIO-TRP1

    Plasmid

    #26881

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    BIO-tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-RGS18HIS-TRP1

    Plasmid

    #26884

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    18xHis tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-TRP1-PGAL1-HBH

    Plasmid

    #26886

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HBH tag N-terminal
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-kanMX6-PGAL1-HBH

    Plasmid

    #26885

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HBH tag N-terminal
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGTRm

    Plasmid

    #127197

    Purpose

    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA PCR template
    

    Use

    Template plasmid for generation of golden gate pc…

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. STAGR_SAMScaffold_hU6

    Plasmid

    #102843

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_hU6promoter
    

    Use

    As pcr template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMH3

    Plasmid

    #52528

    Purpose

    serves as PCR-template to generate HR donors for C-terminal GFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eGFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. STAGR_SAMScaffold_mU6

    Plasmid

    #102845

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_mU6
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMH4

    Plasmid

    #52529

    Purpose

    serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    2x FLAG-tag
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJH131

    Plasmid

    #48260

    Purpose

    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    GAL10/GAL1 bidirectional promoter
    

    Use

    Routine cloning vector

    Tags

    Expression

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    GAL10/GAL1 bidirectional

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge