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pGL2 enhancer- F3 GATA Sp-1 mut
Plasmid#22431DepositorInsertF3 GATA Sp-1 mut (NOS3 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIS57 PGM2 3'UTR
Plasmid#14491DepositorInsertPGM2 3'UTR (PGM2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS56 NETO2 3'UTR
Plasmid#14495DepositorInsertNETO2 3'UTR (NETO2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS55 POGK 3'UTR
Plasmid#14497DepositorInsertPOGK 3'UTR (POGK Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS53 SERP1 3'UTR
Plasmid#14499DepositorInsertSERP1 3'UTR (SERP1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+CMV-Ins-GLuc
Plasmid#89929PurposeExpresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…PromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rInsp-hIns-GLuc
Plasmid#89927PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoter410bp rat insulin II promoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+(rInsp)-Ins-GLuc
Plasmid#89928PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoterrat Insulin II promoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2
Plasmid#174665PurposeExpresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoterDepositorInsertsLUC2
mCherry:T2A:Hygro
UseLentiviral and LuciferaseTagsmCherry and hygromycin dual reporter gene linked …ExpressionMammalianMutationPromoterHuman cytomegalovirus immediate early enhancer/pr…Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
IgK-IFN-luc
Plasmid#14886PurposeReporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expressionDepositorInsertIgK-IFN (IFNB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-IRES
Plasmid#64784PurposeLuciferase reporter vectorDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-Luc
Plasmid#122694PurposeOutput expression of firefly luciferase under the activation of BenR transcription factor for cell-free expressionDepositorInsertFirefly luciferase
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG-FFLuc-3XFLAG
Plasmid#127333PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codonDepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCV40Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-282-LUC
Plasmid#46865Purposemurine 24p3 minimal promoter as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferaseTagsExpressionMutationPromoter24p3 minimal promoterAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/GUG-FFLuc-3XFLAG
Plasmid#127334PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codonDepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mut mod
Plasmid#48165Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from elementDepositorInsert5" UTR of Mut (Mut Mouse)
UseLuciferaseTagsExpressionMutationA/T mutation in the ATG of the uORFPromoterAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-145-LUC
Plasmid#46866Purposemurine 24p3 minimal promoter as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferaseTagsExpressionMutationPromoter24p3 promoter (inactive)Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Kdr
Plasmid#48178Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of 2KDR (MC2R Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN056
Plasmid#220052PurposeReporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).DepositorInsertfLuc-CFTR (exons 22-27) (CFTR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG(30)-FFLuc-3XFLAG
Plasmid#127336PurposeExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30DepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG(59)-FFLuc-3XFLAG
Plasmid#127337PurposeExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59DepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mc2r
Plasmid#48174Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of MC2R (MC2R Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Tas2r3-mod
Plasmid#48175Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> ACG) upstream open reading frame elementDepositorInsertTAS2R3 taste 2 receptor member 3 (TAS2R3 Human)
UseLuciferaseTagsExpressionMutationT/C MUTATION IN ATG OF UORFPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Acsm2 mod
Plasmid#48159Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from elementDepositorInsert5" UTR of Acsm2 (Acsm2 Mouse)
UseLuciferaseTagsExpressionMutationchanged A to T in ATG of uORFPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/Os-ubi:nnLuz-I{PIV-216}:NosT
Plasmid#212191PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the rice ubiquitin (R. ubi) promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterRice ubiquitinAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/MtHp:nnLuz-I{PIV-216}:NosT
Plasmid#212192PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the strong Medicago trunculata MtHP promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterMedicago trunculata MtHP promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/SCBV:nnLuz-I{PIV-216}:NosT
Plasmid#212190PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the Sugarcane bacilliform virus (SCBV) promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterSugarcane Bacilliform VirusAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cluc-RMA
Plasmid#189624PurposeExpresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.DepositorInsertCypridina luciferase fused to Fc
UseAAV and LuciferaseTagsCluc and IgG1 FcExpressionMammalianMutationPromoterhSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Gluc-NHP.RMA-IRES-EGFP
Plasmid#227886PurposeExpresses Gluc-NHP.RMA and EGFP under the hSyn promoter. Gluc-NHP.RMA for monitoring neuronal transduction.DepositorInsertsGaussia luciferase fused to macaque Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianMutationPromoterAvailable sinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS
Plasmid#123199Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInserttMAS::rLUC-I::pMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-Luc
Plasmid#63697Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expressionDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsLuciferaseExpressionMammalianMutationPromoterTATA boxAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP)Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only
