Addgene

We narrowed to 9,506 results for: Gnas

Showing: 1401 - 1450 of 9506 results

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ssGNb-mCherry

Plasmid

#128788

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

ssGNb-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMT p85-GFP

Plasmid

#126660

Purpose

Inducible expression of p85-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

PI3K SH2 containing subunit

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET His10-Src42a

Plasmid

#126674

Purpose

Expression of His10-Src42a for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Src42a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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mTalin2_F3

Plasmid

#111031

Purpose

Plasmid expresses mouse talin2 FERM domain F3

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP and YFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
234_pSKB3-Venus-LUMP

Plasmid

#65975

Purpose

Bacterial expression of Venus-LUMP FRET pair

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Use

Tags

Venus

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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RNb-SNAPf

Plasmid

#128778

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBJ-mPar3

Plasmid

#53230

Purpose

mammalian expression of mouse Par3

Depositor

Shaun Coughlin

Insert

Par3 (F2rl2 Mouse)

Use

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-15 (native signal peptide)

Promoter

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
CMJ119

Plasmid

#69517

Purpose

pBAD eutE1-20- gfpmut2-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGex4T1-JET

Plasmid

#136245

Purpose

Protein expression of Jetlag

Depositor

Aziz Sancar

Article

Ozturk et al J Biol Chem. 2014 Feb 21;289(8):4634-42.

Insert

Jetlag

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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RNb-FRB

Plasmid

#128780

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GNb-FRB

Plasmid

#128772

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-Ns4A-tv-HCVp-P3

Plasmid

#179674

Purpose

TVMVP-repressible ER-associated HCVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

HCVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-SEAP

Plasmid

#179908

Purpose

Secreted Embryonic Alkaline Phosphatase

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pME-6MTnlstdTomato2A

Plasmid

#67685

Purpose

Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

tdTomato

Use

Tags

2A and 6xMyc-NLS

Expression

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-PM mir shLEF1 b

Plasmid

#27018

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 1b

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2deltaST1

Plasmid

#22632

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Aug 31. ():.

Insert

Ror2 delta ST1 (Ror2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST1 domain

Promoter

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
mTalin2_R9

Plasmid

#111042

Purpose

Plasmid expresses the talin2 rod domain R9 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaST2

Plasmid

#22633

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Aug 31. ():.

Insert

Ror2 delta ST2 (Ror2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST2 domain

Promoter

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
Ir25a_GAL80

Plasmid

#83259

Purpose

Expresses GAL80 under the control of Ir25a enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

Ir25a enhancer

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-Notch5#29

Plasmid

#22415

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

Notch5

Use

Riboprobe

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
RNb-zdk1

Plasmid

#128781

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-zdk1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
kermit-2/pCS2+

Plasmid

#16314

Depositor

Peter Klein

Article

Wu et al Development. 2006 Sep . 133(18):3651-60.

Insert

Kermit 2 (gipc2 Frog)

Use

Vertebrate expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
MSCV-PM mir shHoxB9 b

Plasmid

#27020

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 3b

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
ZP45 Prickle-1

Plasmid

#16897

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
57F03_GAL80

Plasmid

#83254

Purpose

Expresses GAL80 under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTLNXA7

Plasmid

#49033

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

chicken alpha7 signal sequence

Expression

Mutation

Promoter

SP6

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
RCAS(B) cNkx2.5 (CT#801)

Plasmid

#13952

Depositor

Cliff Tabin

Article

Smith et al Development. 2000 Sep . 127(17):3671-81.

Insert

Nkx2.5 (NKX2-5 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pSrRGECO1

Plasmid

#222940

Purpose

Expresses RGECO1 (fluorescent calcium indicator) and pac (puromycin resistance) in the Choanoflagellate, Salpingoeca rosetta

Depositor

Pawel Burkhardt

Article

Colgren et al Sci Adv. 2025 Jan 10;11(2):eadr7434. doi

Insert

RGECO1

Use

Salpingoeca rosetta expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-SEAP

Plasmid

#231212

Purpose

Secreted Embryonic Alkaline Phosphatase

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pVG329

Plasmid

#227771

Purpose

For genomic replacement of YOR1 gene in budding yeast

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al bioRxiv 2024.02.28.582517

Insert

Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

N/A

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pVG143

Plasmid

#227754

Purpose

Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al bioRxiv 2024.02.28.582517

Insert

EL222 codon-optimized for budding yeast

Use

Tags

Expression

Yeast

Mutation

Leu87Ile

Promoter

PGK1pr

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p103DestTol2pA2-4xU6sgRNA

Plasmid

#227778

Purpose

Destination vector with U6 driven 4 sgRNAs

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

4xU6:sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
7xTCF-SEAP

Plasmid

#221778

Purpose

SEAP reporter vector for optochemoWnt system

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

7xTCF-SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-H2b-Che

Plasmid

#179650

Purpose

mCherry co-transfection marker

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-p450-P3-cTVMVP

Plasmid

#179664

Purpose

ER-associated uncaged-cTVMVP with P3 leucine zipper

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

cTVMVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RNb-SEpHluorin

Plasmid

#128785

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-SEpHluorin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ssRNb-GFP

Plasmid

#128791

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

ssRNb-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_Neck

Plasmid

#111032

Purpose

Plasmid expresses the unstructured neck region of mouse talin2

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_R5

Plasmid

#111037

Purpose

Plasmid expresses the talin2 rod domain R5 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_F1

Plasmid

#111029

Purpose

Plasmid expresses mouse talin2 FERM domain F1

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_F0

Plasmid

#111028

Purpose

Plasmid expresses mouse talin2 FERM domain F0

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_R6

Plasmid

#111038

Purpose

Plasmid expresses the talin2 rod domain R6 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_R1

Plasmid

#111033

Purpose

Plasmid expresses the talin2 rod domain R1 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_F2

Plasmid

#111030

Purpose

Plasmid expresses mouse talin2 FERM domain F2

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTalin2_R8

Plasmid

#111040

Purpose

Plasmid expresses the talin2 rod domain R8 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CMJ131

Plasmid

#69520

Purpose

pBAD HochAld1-20- gfpmut2- ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CMJ205

Plasmid

#69528

Purpose

pTET pduP1-18-mCherry-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CMJ206

Plasmid

#69529

Purpose

pTET pduD1-20-mCherry-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CMJ137

Plasmid

#69521

Purpose

pBAD etuAld+Ile1-21-gfpmut2- ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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