Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,264 results for: crispr cas9 expression plasmids

Clear keywords
Showing: 561 - 580 of 5264 results

  1. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187956
    Purpose
    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187956-plasmid-map-sequence-id-373523

    Enlarge

  2. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190034-plasmid-map-sequence-id-371641

    Enlarge

  4. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

    Enlarge

  5. BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

    Plasmid
    #101192
    Purpose
    Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K918A/V922A/R925A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101192-plasmid-map-sequence-id-194255

    Enlarge

  6. MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A

    Plasmid
    #101188
    Purpose
    Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    T657A/G658A/W659A/R661A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101188-plasmid-map-sequence-id-194251

    Enlarge

  7. MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

    Plasmid
    #101190
    Purpose
    Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    F491A/M495A/T496A/N497A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101190-plasmid-map-sequence-id-194253

    Enlarge

  8. MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A

    Plasmid
    #101184
    Purpose
    Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Mutation
    G582A/V583A/E584A/D585A/N588A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101184-plasmid-map-sequence-id-194248

    Enlarge

  9. MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

    Plasmid
    #101179
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101179-plasmid-map-sequence-id-194003

    Enlarge

  11. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid
    #187954
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187954-plasmid-map-sequence-id-373498

    Enlarge

  13. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

    Plasmid
    #101181
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/H698A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101181-plasmid-map-sequence-id-194030

    Enlarge

  14. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

    Plasmid
    #101182
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/Q695A/H698A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101182-plasmid-map-sequence-id-194031

    Enlarge

  15. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

    Plasmid
    #101183
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    M694A/Q695A/H698A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101183-plasmid-map-sequence-id-194032

    Enlarge

  16. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

    Plasmid
    #101180
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101180-plasmid-map-sequence-id-194005

    Enlarge

  17. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Article
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

    Enlarge

Showing: 561 - 580 of 5264 results