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We narrowed to 11,832 results for: NSI;

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Showing: 11561 - 11600 of 11832 results

  1. pBOB-Gpr45-Del1&2-3xFlag

    Plasmid
    #245981
    Purpose
    Express 3xFlag C-terminal tagged mouse GPR45-Del1&2 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.
    Insert
    Gpr45 (Gpr45 Mouse)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Del1&2, deletion of R223-D237 and R247-D259
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245981-plasmid-map-sequence-id-488712

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  2. pBOB-Gpr45-Del2-3xFlag

    Plasmid
    #245980
    Purpose
    Express 3xFlag C-terminal tagged mouse GPR45-Del2 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.
    Insert
    Gpr45 (Gpr45 Mouse)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Del2, deletion of R247-D259
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245980-plasmid-map-sequence-id-488693

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  3. pBOB-Gpr45-Del1-3xFlag

    Plasmid
    #245979
    Purpose
    Express 3xFlag C-terminal tagged mouse GPR45-Del1 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.
    Insert
    Gpr45 (Gpr45 Mouse)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Del1, deletion of R223-D237
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245979-plasmid-map-sequence-id-488690

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  7. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233373
    Purpose
    EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_on-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233373-plasmid-map-sequence-id-466778

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  10. pcs2-cDELE1(142-515)-GFP-IRES-mCherry

    Plasmid
    #226102
    Purpose
    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    DELE1 (DELE1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    only encodes for residues 142-515 of DELE1 corres…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226102-plasmid-map-sequence-id-448403

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  11. pTFG001-ACE2(WT)-HA

    Plasmid
    #191569
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available Since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191569-plasmid-map-sequence-id-441362

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  12. AGTR2-DuET

    Plasmid
    #213184
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AGTR2 (AGTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213184-plasmid-map-sequence-id-431748

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  14. pHcRed-NPM1cOligodN243-C1

    Plasmid
    #131847
    Purpose
    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection
    Depositor
    Mark Minden
    Article
    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:
    Insert
    NPM1 (NPM1 Human)
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    This is a deletion contruct of the cytoplasmic m…
    Available Since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    131847-plasmid-map-sequence-id-264401

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  15. pHcRed-NPM1cNLSdN243-C1

    Plasmid
    #131848
    Purpose
    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection
    Depositor
    Mark Minden
    Article
    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:
    Insert
    NPM1 (NPM1 Human)
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    This is a deletion contruct of the cytoplasmic m…
    Available Since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    131848-plasmid-map-sequence-id-264370

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  17. pcDNA3-sACE2v5

    Plasmid
    #145152
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145152-plasmid-map-sequence-id-292590

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  19. pABD933

    Plasmid
    #67917
    Purpose
    Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    CTG10
    Use
    Gateway cloning vector
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

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  21. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  23. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  24. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  26. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    atf4 (ATF4 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226105-plasmid-map-sequence-id-448414

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  29. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Retroviral 5' LTR promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

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  32. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  36. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid
    #197566
    Purpose
    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    Tags
    None
    Expression
    Mammalian
    Mutation
    N166A, V168G, W239C
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197566-plasmid-map-sequence-id-388228

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  37. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

    Plasmid
    #111394
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    GCaMP6f
    Use
    AAV
    Tags
    6xHis, Myc, T7, and Xpress
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    111394-plasmid-map-sequence-id-212394

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  38. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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Showing: 11561 - 11600 of 11832 results